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RNA-sequencing of wild-type and IRF4-deficient splenic cDC2s

ABSTRACT: RNA-sequencing of wild-type and IRF4-deficient splenic cDC2s

PROVIDER: PRJNA780492 | ENA |

REPOSITORIES: ENA

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RNA-sequencing of wild-type and IRF4-deficient splenic cDC2s

Project description:We analyzed the transcriptome differences of wild-type and IRF4-deficient (CD11c-cre x Irf4fl/fl) type-2 conventional dendritic cells (cDC2s) in spleen. Wild-type or IRF4-deficient (CD11c-cre x Irf4fl/fl) bone marrow cells were transferred into lethal irradiated CD45.1 mice. 8 weeks later, splenic cDC2s were purified and prepared for RNA-seq analysis. Three technical repeats of ~10^5 cells per sample from each of one mouse were included. CD97 was downregulated in IRF4-deficient cDC2s. GSEA showed that upregulated and downregulated genes in CD97-deficient cells were strongly enriched in IRF4-regulated genes. These data are in accord with CD97 acting within the IRF4 gene-expression network.

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RNA-sequencing of wild-type and ArhGEF1-deficient splenic cDC2s

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RNA-sequencing of wild-type and ArhGEF1-deficient splenic cDC2s

Project description:RNA-sequencing of wild-type and ArhGEF1-deficient splenic cDC2s

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RNA-sequencing of wild-type, CD97- and Gα13-deficient splenic cDC2s

Project description:RNA-sequencing of wild-type, CD97- and Gα13-deficient splenic cDC2s

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Transcription profiling by array of mouse irf4 knockout mature splenic B cells against wild type controls from littermates

Project description:Gene expression profiling of murine irf4-/- and irf4+/+ splenic B cells identifies genes regulated by the transcription factor IRF4 in quiescent mature B cells. B cells from 12-week old irf4-/- and irf4+/+ littermate mice were isolated by magnetic depletion of non-B cells from splenic mononuclear cells. RNA was isolated. Labeled cRNA was hybridized to microarrays and genes specifically expressed in irf4-/- vs. irf4+/+ samples.

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