Comparison between blue and green Spanish firs
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ABSTRACT:
PROVIDER: PRJNA781601 | ENA |
REPOSITORIES: ENA
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Project description:Spanish fir (Abies pinsapo) is a relict tree that is found in southern Spain mountains. In natural conditions two phenotypes can be observed, trees with blue needles and trees with green needles. The aim of the present work is to elucidate at transcriptomic levels the possible causes of the differences between these phenotypes.
2021-11-20 | GSE189122 | GEO
Project description:We have studied the transcriptional, metabolic and photo-physiological responses to light of different spectral quality in the marine diatom Phaeodactylum tricornutum through time-series studies of cultures exposed to equal doses of photosynthetically usable radiation of blue, green and red light. The experiments showed that short-term differences in gene expression and profiles are mainly light quality-dependent. Transcription of photosynthesis-associated nuclear genes was activated mainly through a light quality-independent mechanism likely to rely on chloroplast-to-nucleus signaling. In contrast, genes encoding proteins important for photoprotection and PSII repair were highly dependent on a blue light receptor-mediated signal. Changes in energy transfer efficiency by light-harvesting pigments were spectrally dependent; furthermore, a declining trend in photosynthetic efficiency was observed in red light. The combined results suggest that diatoms possess a light quality-dependent ability to activate photoprotection and efficient repair of photodamaged PSII. In spite of approximately equal numbers of PSII-absorbed quanta in blue, green and red light, the spectral quality of light is important for diatom responses to ambient light conditions.
2014-12-22 | GSE55959 | GEO
Project description:We have studied the transcriptional, metabolic and photo-physiological responses to light of different spectral quality in the marine diatom Phaeodactylum tricornutum through time-series studies of cultures exposed to equal doses of photosynthetically usable radiation of blue, green and red light. The experiments showed that short-term differences in gene expression and profiles are mainly light quality-dependent. Transcription of photosynthesis-associated nuclear genes was activated mainly through a light quality-independent mechanism likely to rely on chloroplast-to-nucleus signaling. In contrast, genes encoding proteins important for photoprotection and PSII repair were highly dependent on a blue light receptor-mediated signal. Changes in energy transfer efficiency by light-harvesting pigments were spectrally dependent; furthermore, a declining trend in photosynthetic efficiency was observed in red light. The combined results suggest that diatoms possess a light quality-dependent ability to activate photoprotection and efficient repair of photodamaged PSII. In spite of approximately equal numbers of PSII-absorbed quanta in blue, green and red light, the spectral quality of light is important for diatom responses to ambient light conditions. Continuous, axenic culturing of P. tricornutum was done as described in Nymark et al. (2009). The cultures were incubated at 15M-BM-0C under cool white fluorescent light (Philips TLD 36W/96) providing a scalar irradiance (EPAR) of 100 M-NM-<mol m-2 s-1 under continuous white light (CWL) conditions. Upon the onset of the experiment the cultures were synchronized by 48 h dark-treatment (D48). Thereafter the algae were exposed to blue light (BL), green light (GL) or red light (RL) provided by a waveband specific LED panel (SL3500, Photon Systems Instruments). The algae were exposed to 0.5 h, 6 h or 24 h of: 1) 230 M-NM-<mol m-2 s-1 of RL, 2) 100 M-NM-<mol m-2 s-1 of GL, 3) 50 M-NM-<mol m-2 s-1 of BL or 4) 100 M-NM-<mol m-2 s-1 of WL respectively. Three biological replicas for each of the treatments were harvested. Data for the white light exposure and 48h dark treatment is described in GSE42039, PMID: 23520530.
2014-12-22 | E-GEOD-55959 | biostudies-arrayexpress
Project description:The purpose of this study is to investigate whether LED light at different wavelength can improve wound healing in normal fibroblast. In this study, both diabetic and normal fibroblast cell lines were cultured and created some artificial wounds with pipette tips. After LED irradiation and microarray analysis, we found that GPCR Class A, a rhodopsin-like-structure gene, was significantly up regulated in all treated groups. Besides, for normal cell treated groups, the expression of other genes relevant to defense response to virus became strongly higher. However, in diabetic cells, genes relating to acute inflammatory response and mitotic cell cycle were highly expressed. Researchers also found that diabetic cells significantly respond to wound healing better than normal cells because inflammatory response, cell migration, cell proliferation, cell adhesion, and regulation of mitosis pathways can be instantly activated by red and green LED light according to gene expression profiles. Normal cell lines were irradiated with different wavelength (red/green/blue) LED color and compare their gene expression with the control groups.
2016-02-18 | E-GEOD-78018 | biostudies-arrayexpress
Project description:The purpose of this study is to investigate whether LED light at different wavelength can improve wound healing in both diabetic fibroblast. In this study, both diabetic and normal fibroblast cell lines were cultured and created some artificial wounds with pipette tips. After LED irradiation and microarray analysis, we found that GPCR Class A, a rhodopsin-like-structure gene, was significantly up regulated in all treated groups. Besides, for normal cell treated groups, the expression of other genes relevant to defense response to virus became strongly higher. However, in diabetic cells, genes relating to acute inflammatory response and mitotic cell cycle were highly expressed. Researchers also found that diabetic cells significantly respond to wound healing better than normal cells because inflammatory response, cell migration, cell proliferation, cell adhesion, and regulation of mitosis pathways can be instantly activated by red and green LED light according to gene expression profiles. Diabetic fibroblast cells were irradiated with different wavelength (red/green/blue) LED color and compare their gene expression with the control groups.
2016-02-18 | E-GEOD-78017 | biostudies-arrayexpress
Project description:Comparison for gene expression of Acinetobacter baumannii Lab-WT under blue light irradiation
2022-01-31 | GSE195643 | GEO
Project description:We investigated a novel, simple approach to induce the production of cryptic secondary metabolites in actinomycetes by stimulating the organism with high-intensity monochromatic green light (180 radiation unit). Streptomyces coelicolor A3(2) produces blue antibiotic actinorhodin (ACT) and red antibiotic undecylprodigiosin (RED). Using these two pigment antibiotics as indicators, we found that sporulation acceleration and regulation of the antibiotic production pathways can be induced by using high-intensity monochromatic green LEDs. Therefore, we investigated the immediate response of S. coelicolor A3(2) gene expression to the strong green LED stimulation.
2023-12-10 | E-MTAB-12876 | biostudies-arrayexpress
Project description:Library comparison between TruSeq, SMARTer and TeloPrime
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Project description:Whole genome comparison between Erhlichia chaffeensis strains
| PRJNA97595 | ENA