Transcriptomic profiles of jejunum, liver, spleen and jejunal Peyer's patches in piglets exposed to Fumonisin B1
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PROVIDER: PRJNA781945 | ENA |
REPOSITORIES: ENA
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Project description:Fumonisin B1 (FB1) is a mycotoxin produced by Fusarium species. In mammals, this toxin causes multiple organ-specific damages. Among its multiple effects, FB1 promotes hepatotoxicity, is immunotoxic and alters the intestinal functions. Despite its inhibitory effect on de novo ceramide synthesis, the molecular mechanism of FB1 action and toxicity remain unclears. In order to explore the mechanism of FB1 toxicity in multiple tissues, we analyzed the transcriptome of the jejunal section, liver, spleen and jejunal Peyer's patches which are targeted by FB1.
2022-03-02 | GSE189198 | GEO
Project description:Fumonisin B1 (FB1) is a mycotoxin produced by Fusarium species. In mammals, this toxin causes multiple organ-specific damages. Among its multiple effects, FB1 promotes hepatotoxicity, is immunotoxic and alters the intestinal functions. Despite its inhibitory effect on de novo ceramide synthesis, the molecular mechanism of FB1 action and toxicity remain unclears. In order to explore the mechanism of FB1 toxicity, we analyzed the transcriptome of the jejunal Peyer's patches.
2019-04-15 | GSE97819 | GEO
Project description:To establish better understanding of cells found in jejunal and ileal Peyer's patches of pigs, we utilized single-cell RNA sequencing scRNA-seq and spatial transcriptomics to recover and analyze cells and spatial regions from sections of jejunum and ileum containing Peyer's patches. Cells identified via single-cell RNA sequencing included B, T/innate lymphoid cell, myeloid, epithelial, and stromal lineage cells. Spatial dots recovered via spatial transcriptomics belonged to regions including villi, crypts, interfollicular/parafollicular zones, follicles, and muscularis. Overall, results provide new information on regional localization and transcriptional profiles of cells in the pig small intestine.
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Project description:The Toxoplasma type I ROP16 kinase directly activates the host STAT3 and STAT6 transcription factors and when transgenically expressed in the orally virulent type II strain, promotes host resistance to oral challenge. The transcriptional profile of type II and II+ROP16I infected Peyer's patches and intestines from orally infected mice on day 5 was determined to elucidate host signaling pathways and molecular gene targets that correlate with protective immunity in the gut of orally challenged animals C57BL/6 female mice were orally gavaged with 1000 tissue cysts of the type II or II+ROP16I strain. On Day 5 of infection the Peyer's patches and intestines were analyzed for parasite burden by bioluminescence imaging. Individual Peyer's patches and intestinal sections, corresponding to the middle third of the intestine (~jejunum), that had equivalent parasite burden were snap frozen in liquid nitrogen. The samples were crushed using a sterile 16 G needle in tubes on dry ice. In the case of the intestine, the sample was resuspended in TRizol, extruded through a needle using a syringe and RNA was isolated according to manufacturer's protocol; for the Peyer's patch, the sample was suspended in cell lysis buffer and RNA isolated using the RNeasey kit (Invitrogen) according to the manufacter's protocol.
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Project description:The purpose of this experiment is to identify the molecules which are regulated by epithelial RANK in Peyer's patches.
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Project description:This study couples a swine experimental model of non-typhoid salmonellosis to laser capture microdissection and microarray analysis to dissect the mechanisms carried out in the follicles of ileum Peyer’s Patches in response to Salmonella Typhimurium infection. Affymetrix GeneChip® Porcine genome array was used to study the gene expression profiles of Peyer's Patches grom control and infected pigs at acute phase of infection.
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Project description:Fumonisin B1 (FB1) is a mycotoxin produced by Fusarium species. In mammals, this toxin causes multiple organ-specific damages. Among its multiple effects, FB1 promotes hepatotoxicity, is immunotoxic and alters the intestinal functions. Despite its inhibitory effect on de novo ceramide synthesis, the molecular mechanism of FB1 action and toxicity remain unclears. In order to explore the mechanism of FB1 toxicity, we analyzed the transcriptome of the jejunal section which is targeted by FB1.
2019-04-15 | GSE97817 | GEO
Project description:Transcriptomic profiles of jejunal Peyer's patches in piglets exposed to Fumonisin B1
| PRJNA382967 | ENA
Project description:Many studies have shown that the mucous membranes and skin are at the interface with different external environments and face the disparity of pathogenic effects, such as biological agents, chemical or physical environment. This difference may demand distinct immune responses. However, the mechanism to induce the distinct immune responses in mucous and skin is largely unknown. Dendritic cells of mucosa and skin are crucial in the initiation of immune responses, maintenance of self-tolerance and antigens presentation T cells. The different functions between mucosal and epidermal dendritic cells may play an important role in different immune responses. To compare the different gene expression of the mucosal DC and Langerhans cells (LC), we utilized microarrays to investigate different gene expression profiles in mucosal DC isolated from PPs (PDC) and epidermal LC from skin (ELC). 3548 genes were shown to be differentially expressed between PDC and ELC. According to genes annotations, 105 genes may be involved in immunity process. The genes involved in immune process were categorized to five groups related to DC function, including antigen presentation, antigen uptake, cytokines chemokines, and receptors, cell surface molecules and signal transduction. 11 of the highest expressed genes were selected as the candidate genes and reformed by real-time PCR. These 11 selected genes might be suitable candidates to further study the difference of gene expression between mucosal DC and epidermal LC and would be used for design for new vaccine. Beads and FACS sort were used to isolate dendritic cells from Peyer's patches and epidermal of skin from 80 four-week-old female BALB/c mice. Dendritic cells from Peyer's patches were pooled as one sample, and dendritic cells from epidermal of skin were pooled as another sample. RNA isolation, amplification, cDNA labelling, microarray hybridization and analyses were performed according to the Affymetrix manual book.
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Project description:To identify gene products expressed in S100A4-producing cells in Peyer's patches, we used transgenic mice expressing enhanced green fluorescent protein (EGFP) under the control of the mouse S100A4 promoter (S100A4-GFP mice) and analyzed gene expression profiles using microarray. As a result, we found that S100A4-producing cells expressed many genes characteristic of LysoMac/DCs and ILC3s.
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