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RNASeq to investigate SOX2 and SOX9 function in human fetal lung tip progenitor cells

ABSTRACT: RNASeq to investigate SOX2 and SOX9 function in human fetal lung tip progenitor cells

PROVIDER: PRJNA784953 | ENA |

REPOSITORIES: ENA

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RNASeq to investigate SOX2 and SOX9 function in human fetal lung tip progenitor cells

Project description:In order to understand transcription factor SOX2 and SOX9 function, we have knocked down SOX2 and SOX9 in human fetal lung tip progenitor organoids using an inducible CRISPRi system. We discovered that SOX2 knockdown didn't appear to influence organoid cell survival and led to minimal gene expression changes at transcriptome level. By contrast, SOX9 knockdown led to organoid cell self-renewal defects and we have detected hundreds of genes differentially expressed after SOX9 knockdown.

2022-04-30 | GSE189884 | GEO

SOX9+/PTF1A+ Cells Define the Tip Progenitor Cells of the Human Fetal Pancreas of the Second Trimester

Project description:Significant progress has been made in recent years in characterizing human multipotent progenitor cells (hMPCs) of the early pancreas; however, the identity and persistence of these cells during the second trimester, after the initiation of branching morphogenesis, remain elusive. Additionally, studies on hMPCs have been hindered by few isolation methods that allow for the recovery of live cells. Here, we investigated the tip progenitor domain in the branched epithelium of human fetal pancreas between 13.5 and 17.5 gestational weeks by immunohistological staining. We also used a novel RNA‐based technology to isolate live cells followed by gene expression analyses. We identified cells co‐expressing SOX9 and PTF1A, two transcription factors known to be important for pancreatic MPCs, within the tips of the epithelium and observed a decrease in their proportions over time. Pancreatic SOX9+/PTF1A+ cells were enriched for MPC markers, including MYC and GATA6. These cells were proliferative and appeared active in branching morphogenesis and matrix remodeling, as evidenced by gene set enrichment analysis. We identified a hub of genes pertaining to the expanding tip progenitor niche, such as FOXF1, GLI3, TBX3, FGFR1, TGFBR2, ITGAV, ITGA2, and ITGB3. YAP1 of the Hippo pathway emerged as a highly enriched component within the SOX9+/PTF1A+ cells. Single‐cell RNA‐sequencing further corroborated the findings by identifying a cluster of SOX9+/PTF1A+ cells with multipotent characteristics. Based on these results, we propose that the SOX9+/PTF1A+ cells in the human pancreas are uncommitted MPC‐like cells that reside at the tips of the expanding pancreatic epithelium, directing self‐renewal and inducing pancreatic organogenesis.

2019-11-27 | GSE141087 | GEO

Expression data from mouse embryonic lung epithelial tip progenitor cells

Project description:Epithelial tip progenitor cells are an important epithelial progenitor population in the developing lung. At early stages of development they produce SOX2+ bronchiolar progenitor cells. At later stages of embryonic lung development they produce SOX2- alveolar progenitor cells. We purified the tip progenitor cells from early (E11.5, bronchiolar progenitor producing) and late (E17.5, alveolar progenitor producing) stages of mouse lung development and used Affymetrix microarrays to compare their gene expression profiles.

2016-08-31 | GSE75860 | GEO

Human fetal lung tip progenitor cells

Project description:Human fetal lung tip progenitor cells

| PRJNA784950 | ENA

SOX9+/PTF1A+ Cells Define the Tip Progenitor Cells of the Human Fetal Pancreas of the Second Trimester

Project description:SOX9+/PTF1A+ Cells Define the Tip Progenitor Cells of the Human Fetal Pancreas of the Second Trimester

| PRJNA592003 | ENA

RNASeq to investigate ETV4 and ETV5 function in human foetal lung tip progenitor cells

Project description:In order to understand transcription factor ETV4 and ETV5 function, we have knocked down ETV4 and ETV5 synergistically in human foetal lung tip progenitor organoids using an inducible CRISPRi system. We discovered that ETV double knockdown led to organoid cell self-renewal defects

2022-07-02 | GSE207179 | GEO

RNASeq to investigate ETV4 and ETV5 function in human foetal lung tip progenitor cells

Project description:RNASeq to investigate ETV4 and ETV5 function in human foetal lung tip progenitor cells

| PRJNA854069 | ENA

SOX9+/PTF1A+ Cells Define the Tip Progenitor Cells of the Human Fetal Pancreas of the Second Trimester.

Project description: Not available

| S-EPMC6877773 | biostudies-literature

ChIP-seq on Olig2, Ascl1, Tcf3, Max, NFI, Sox2, Sox9 and Sox21 in mouse neural stem cells

Project description:Definition of genome wide binding profile of Olig2, Ascl1, Tcf3, Max, NFI, Sox2, Sox9 and Sox21 in mouse neural stem cells. Three sets of processed data files, together with a README file (README_E-MTAB-2228.docx) describing the source and content of each file, are provided as additional files to this ArrayExpress submission and can be found in https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-2228 .

2014-09-19 | E-MTAB-2228 | biostudies-arrayexpress

Embryonic mouse mammary progenitor cells +/-Sox9

Project description:CRISPR/Cas9-mediated gene targeting of Sox9 from embryonic mammary progenitors cell line eMPC1. Part of a study to assess the effect of Sox9 ablation on embryonic mammary progenitor cell fate and function.

2018-11-30 | E-MTAB-6846 | biostudies-arrayexpress

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