Ontology highlight
ABSTRACT:
PROVIDER: PRJNA785863 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The big brown bat has an exceptionally long lifespan
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Project description:Brown adipose tissue (BAT) is a thermogenic organ that protects animals against hypothermia and obesity. BAT derives from the multipotent paraxial mesoderm; however, the identity of embryonic brown fat progenitor cells and regulators of adipogenic commitment are unclear. We identified the transcription factor GATA6 as a selective marker of brown adipogenic progenitor cells. Deletion of Gata6 in the brown fat lineage resulted in a striking loss of BAT. To gain insight into the mechanism by which GATA6 supports BAT development, we performed ChIP-seq for GATA6 from the BAT of embryonic day 15.5 embryos.
2023-07-19 | GSE234012 | GEO
Project description:PR (PRD1-BF1-RIZ1 homologous) domain-containing 16 (PRDM16) drives a brown fat differentiation program, but the mechanisms by which PRDM16 activates brown fat-selective genes have been unclear. Through chromatin immunoprecipitation (ChIP) followed by deep sequencing (ChIP-seq) analyses in brown adipose tissue (BAT), we reveal that PRDM16 binding is highly enriched at a broad set of brown fat-selective genes. Importantly, we found that PRDM16 physically binds to MED1, a component of the Mediator complex, and recruits it to superenhancers at brown fat-selective genes. PRDM16 deficiency in BAT reduces MED1 binding at PRDM16 target sites and causes a fundamental change in chromatin architecture at key brown fat-selective genes. Together, these data indicate that PRDM16 controls chromatin architecture and superenhancer activity in BAT.
2015-02-10 | GSE63964 | GEO
Project description:We performed a genome-wide deep sequencing analysis of the microRNAs abundant in mesenchymal stem cells (MSCs) derived from murine brown adipose tissue and in in vitro differentiated mature brown adipocytes. Several microRNAs were identified as differentially regulated when comparing datasets from MSCs vs. mature fat cells. These microRNAs may have an implication in the regulation of adipogenesis as well as thermogenesis in brown adipose tissue (BAT). Examination of BAT-derived MSCs (BAT-MSC; 1 sample) and in vitro differentiated mature brown fat cells (BAT-DIFF; 1 sample) vertis biotechnologie AG, D-85354 Freising, Germany (library construction and sequencing)
2013-05-28 | E-GEOD-45499 | biostudies-arrayexpress
Project description:Brown adipose tissue (BAT) has metabolic and endocrine effects to the whole-body, secreting molecules that target different tissues. Using transcriptomic analysis from isolated BAT from mice treated with isoproterenol in WT or UCP1-knockout mice, which have disfunctional BAT, we identified Bmp3b as an adipokine produced and secreted by activated BAT.
2023-08-01 | GSE210613 | GEO
Project description:Using high throughput sequencing we report chromatin accessibility(ATAC-seq) and transcriptome profiling (RNA-seq)and in mouse brown adipose tissue (BAT) upon cold exposure in wildtype and Dot1L-BAT specific KO mice.
2020-11-10 | GSE159645 | GEO
Project description:EBF2 activates the expression of brown fat-selective genes in adipocytes, but whether EBF2 regulates these genes via direct binding was unknown. To address this question, we analyzed the genome-wide binding profile of EBF2 in BAT using chromatin immunoprecipitation followed by deep sequencing (ChIP-seq). To determine if EBF2 is required for the activity of lineage-specific enhancers, we examined the levels of PPARγ, RNA Polymerase II (Pol II), and H3K27ac at brown fat-specific genes in wildtype (WT) and Ebf2 KO BAT.
2017-04-27 | GSE97114 | GEO
Project description:To analyze the gene expression profile of BAT in heart failure mice model, we performed whole genome microarray expression profiling using brown adipose tissue (BAT) from mice at 2 weeks after Sham or TAC operation.
2022-08-31 | GSE167095 | GEO
Project description:Prdm16 is a transcription factor that drives a complete program of brown adipocyte differentiation, but the mechanism by which Prdm16 activates gene transcription remains unknown. Utilizing ChIP-seq teqhnique, we found that Prdm16 binds to chromatin at/near many brown fat-selective genes in BAT. Interestingly, Prdm16-deficiency dramatically reduced the binding of Med1 to Prdm16-target sites. Indeed, Prdm16 binds and recruits Med1 to BAT-enriched genes and the loss of Prdm16 caused a fundamental change in chromatin architecture at key BAT-selective genes and also reduced transcirptional activity. Moreover, Prdm16, through its interaction with Med1, defines and regulates the activity of super-enhancers that drive the expression of cell identity genes. Together, these data demonstrate that Prdm16 drives gene transcription by recruiting Med1 to control chromatin architecture and super-enhancers. Brown adipose tissues were collected from Prdm16 knockout and wiletype 9-month-old mice and ChIP-seq was performed for Prdm16, PolII, Med1, and H3K27ac.
2015-02-10 | E-GEOD-63965 | biostudies-arrayexpress