Project description:Effect of CaM overexpression on Arabidopsis transcriptome. Unlike animals, plants are immobile and cannot simply move away from unfavourable environments and thus have developed complex mechanisms to respond to and sense biotic and abiotic signals. These stimuli often lead to tightly controlled changes in cytoplasmic free calcium concentration [Ca2+]cyt termed "calcium signatures" which are thought to be, at least partly, responsible for the specificity of plant responses to the environment. However little is known about how exactly these calcium signatures are decoded into specific end-responses. Calmodulin (CaM) is the most well characterised Ca2+ binding protein and is the primary sensor of changing [Ca2+]. Upon binding Ca2+ CaM undergoes a conformational change allowing binding and activation of a wide variety of target proteins. In plants CaM exists in gene families encoding multiple isoforms. The expression of individual CaM genes can be differentially regulated and isoforms may be differentially localised. Furthermore specific isoforms can bind and activate different target proteins. These features of plant CaM allow the possibility of specificity during calcium signalling in response to specific stimuli. The effect of overexpression of four CaM protein isoforms on the Arabidopsis thaliana transcriptome will be investigated. Ten day old transgenic Arabidopsis seedlings (containing estradiol inducible CaM overexpression constructs) were induced for 9hrs in 5uM estradiol with appropriate water (0.025% DMSO) and empty vector controls.

2008-02-15 | E-NASC-65 | biostudies-arrayexpress

Transcript and Metabolite Changes during the Early Phase of ABA-mediated Induction of CAM in Talinum triangulare

Project description:Crassulacean Acid Metabolism (CAM) has evolved as a water saving strategy and its engineering into crops offers an opportunity to improve their water-use efficiency. This requires a comprehensive understanding of the regulation of the CAM pathway. Here, we use the facultative CAM species Talinum triangulare as a model in which CAM can be induced rapidly by exogenous abscisic acid (ABA). RNA-sequencing and metabolite measurements were employed to analyse the changes underlying CAM induction and identify potential CAM regulators. Non-negative matrix factorisation followed by k¬-means clustering identified an early CAM-specific cluster and a late one, which was specific for the early light phase. Enrichment analysis revealed ABA metabolism, WRKY-regulated transcription, sugar and nutrient transport and protein degradation in these clusters. Activation of the CAM pathway was supported by up-regulation of phosphoenolpyruvate carboxylase, cytosolic and chloroplastic malic enzymes and several transport proteins as well as by increased end-of-night titratable acidity and malate accumulation. Transcription factors HSFA2, NF-YA9 and JMJ27 were identified as candidate regulators of CAM induction. With this study we promote the model species T. triangulare, in which CAM can be induced in a controlled way, enabling further deciphering of CAM regulation.

2019-04-11 | GSE116590 | GEO

bacterium AMD02 isolate:AMD02

Project description:bacterium AMD02 isolate:AMD02 Genome sequencing and assembly

| PRJNA512852 | ENA

bacterium G20 isolate:G20

Project description:bacterium G20 isolate:G20 Genome sequencing and assembly

| PRJNA360190 | ENA

Planctomycetes bacterium isolate:bin_134

Project description:Planctomycetes bacterium isolate:bin_134 Genome sequencing and assembly

| PRJNA691170 | ENA

bacterium M21 isolate:M21

Project description:bacterium M21 isolate:M21 Genome sequencing and assembly

| PRJNA360194 | ENA

Gammaproteobacteria bacterium isolate:ljb5

Project description:Gammaproteobacteria bacterium isolate:ljb5 Genome sequencing and assembly

| PRJNA714283 | ENA

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