Project description:Mangrove plants are the demonstrated woody plants in tropical and subtropical coastal area with great ecological and economic importance. However, due to the extreme global climate change and increased human activities, mangroves is suffering a dramatic declining worldwide. Lumnitzera littorea (Jack) Voigt is one of the most endangered mangrove species in China. A comprehensive understanding on its resistance mechanisms to low ambient temperature help us to better conserve and restore L. littorea. In the current study, we performed comparative transcriptome analysis to investigate the genome-wide changes of gene expression profile in L. littorea under chilling stress (8°C day/5°C night) over normal condition (25°C day/23°C night). The low temperature suppressed fatty acid elongation and protein phosphorylation in L. littorea, while induced calcium ion binding process and signaling transduction, suggesting an activation of cold-stress sensing and signaling in L. littorea. Combining our analysis with our previous physiological assays, we showed a substantial photoinhibition occurring in the seedlings of L. littorea with the decrease of ambient temperature, and the synthesis of photosystem II reaction center protein D1 and peroxidase-involved scavenging of reactive oxygen species (ROS) were enhanced accordingly to combat the adverse impacts. Finally, we highlighted the biological significance of post-transcriptional regulation, including RNA binding and surveillance, in coping with cold stress in L. littorea. Collectively, our findings provide a global view to the resistance mechanisms of chilling stress in L. littorea, and valuable genetic resources to assist the protection and restoration of L. littorea.
Project description:To investigate the regulatory mechanisms of GmZF392, GmZF351 and GmNFYA in oil accumulation, seeds at H3 stage of transgenic plants and JACK plants were collected for RNA extraction and sequencing. Before sequencing, qPCR showed that expression of GmZF392, GmZF351 and GmNFYA increased to about 10, 109 and 7 times respectively compared to JACK. Clean reads of RNA-sequencing were mapped to soybean genome and genes that had a 1.5 fold increase or 50% decrease, compared to JACK, were defined as differential expressed genes (DEGs).
Project description:To investigate the regulatory mechanisms of GmNFYA in salt stress, leaves and roots of transgenic plants and JACK plants were collected for RNA extraction and sequencing. Clean reads of RNA-sequencing were mapped to soybean genome and genes that had a 2 fold increase or 50% decrease, compared to JACK, were defined as differential expressed genes (DEGs).
