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Transcriptome sequencing of bristol strain (wild-type), metl-9 KO strain and metl-9 mutation strain upon P.aeruginosa infection

ABSTRACT: Transcriptome sequencing of bristol strain (wild-type), metl-9 KO strain and metl-9 mutation strain upon P.aeruginosa infection

PROVIDER: PRJNA793702 | ENA |

REPOSITORIES: ENA

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Transcriptome sequencing of bristol strain (wild-type), metl-9 KO strain and metl-9 mutation strain upon P.aeruginosa infection

Project description:We challenge bristol strain (wild-type), metl-9 KO strain (short as KO, has a 101bp insertion, leads to a truncated protein of 258aa) and metl-9 catalytic-activity mutated strain (short as mut, has N172K, D274G mutations in full-length protein) with P.aeruginosa (P.A14), and observe a discrepant transcriptome pattern between wild-type and KO/mut strains. Plenty of innate immune response genes show different expression patterns upon P.A14 infection between the wild-type strain and KO/mut strain. It indicates the important role of metl-9 and 6mA in worm innate immune response modulation.

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Project description:Five biological repeats of P.aeruginosa PA14 and five biological repeats of P.aeruginosa PA14 metR::phoA were grown in liquid BM2 swarming media containing 62mM potassium phosphate buffer [pH7], 2 mM MgSO4, 10uM FeSO4, 0.4% (wt/vol) glucose, and 0.1% (wt/vol) casamino acids. Microarray experiments were done using microarray slides and protocols from TIGR on samples taken from mid-log phase (OD600 0.4-0.5)cells.

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Caenorhabditis elegans strain:Bristol (N2)

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Transcription profiling of P.aeruginosa PAO1 and PAO1 phoQ::xylE

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