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Comparison of changes in mRNA expression profiles in arteries and heart valves in rabbit models of dietary lipid-triggered cardiovascular diseases

ABSTRACT: Comparison of changes in mRNA expression profiles in arteries and heart valves in rabbit models of dietary lipid-triggered cardiovascular diseases

PROVIDER: PRJNA794345 | ENA |

REPOSITORIES: ENA

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Comparison of changes in mRNA expression profiles in arteries and heart valves in rabbit models of dietary lipid-triggered cardiovascular diseases

Project description:We report RNA-seq data obtained on dissected aorta and four heart valves from rabbits fed with chow diet (n=7), cholesterol-rich diet (n=6) or lard-rich diet (n=7). Rabbits that received cardiac irradiation combined with the cholesterol-rich diet were also included (n=7).

2022-01-08 | GSE193060 | GEO

Next Generation Sequencing analysis of control and serotonin, pergolide and dexfenfluramine treated tricuspid valves

Project description:Identify biological processes leading to heart valve disease upon long-term oral administration of serotonin, pergolide and dexfenfluramine Transcriptome profiling of tricuspid valves isolated from the drug treateds rabbit groups and compared to control rabbits using RNA sequencing Distinct patterns of differentially expressed genes (DEGs) highlight common and specific biological processes of drug treatment in tricuspid valves

2020-06-29 | GSE113082 | GEO

Human Cardiovascular Tissue Extracellular Vesicles

Project description:Fewer than 50% of patients develop vascular and valvular calcification, implying differential pathogenesis. Tissue-entrapped extracellular vesicles (EVs) are found in mineralization but their contents and functions are unstudied. Tissue EVs were isolated from normal and diseased human carotid arteries and aortic valves by enzymatic digestion, serial (ultra)centrifugation and density-gradient separation.

2023-07-10 | PXD035529 | Pride

Human Cardiovascular Tissue Extracellular Vesicles – Density Gradient

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2023-07-10 | PXD035580 | Pride

Microarray-based gene expression of rabbit basilar artery smooth muscle cells exposed to hemodynamic insult

2016-06-30 | GSE61212 | GEO

Human Cardiovascular Tissue Extracellular Vesicles – Ultracentrifugation vs. Density Gradient

2023-07-10 | PXD035485 | Pride

Human Cardiovascular Tissue Extracellular Vesicles – Iodixanol Mass Exclusion

2023-07-10 | PXD035505 | Pride

Microarray-based gene expression of rabbit basilar artery smooth muscle cells exposed to hemodynamic insult

Project description:High-flow causes the remodeling of arteries, in which smooth muscle cells play an important role. To know the profile of smooth muscle gene expression under high-flow conditions in vivo, flow of rabbit basilar artery was increased by ligation of both common carotid arteries. Microarrays were performed to profile the gene expression of smooth muscle cells isolated from rabbit basilar artery. Expression profiles indicate 43603 differentially expressed genes in smooth muscle cells exposed to high-flow insult compared with the sham control, of which 1470 genes were upregulated and 780 genes downregulated using 2 fold-changes and P<0.05 as a cut-off. Bilateral common carotid arteries of female New Zealand White rabbits were ligated to increase vascular flow.The control group was performed the same procedure to expose the CCAs without ligation. Rabbits were euthanized at day 5 after ligation or exposure of bilateral CCAs in both groups (n=3 for each group). The rabbits used and all procedures in this study were approved by the local Institutional Animal Care and Use Committee. Smooth muscle cells were isolated. After euthanization of rabbits, the whole basilar arteries were removed. The arteries were cleaned in PBS buffer,cannulated and perfused at a constant flow with a cocktail which contains PBS and 0.4 mg/ml elastase (Sigma) and 1 mg/ml collagenase (type 1A, Sigma). After an incubation time of 45 min, the tissue left was removed and stored in PBS. SMCs were released from the artery by trituration. Then Total RNA was extracted and gene chip tests were performed.

2016-06-30 | E-GEOD-61212 | biostudies-arrayexpress

Rabbit Lung_DH_TO_Control

Project description:Rabbit Lung_DH_TO_Control

| PRJEB9024 | ENA

Microarray-based gene expression on human aortic valves

Project description:Aortic valves are collected from patients with severe aortic valve stenosis undergoing aortic valve replacement at the Institut universitaire de cardiologie et de pneumologie de Québec (IUCPQ), Quebec City, Canada. From this biobank collection, transcriptomic analyses from 240 aortic valves were performed. All valves were tricuspid and had a fibro-calcific remodeling score of 3 or 4. RNA was extracted from valve leaflets and gene expression evaluated using the Illumina HumanHT-12 v4 Expression BeadChip. The main objective of this study was to perform a large-scale expression quantitative trait loci (eQTL) mapping study on human aortic valves.

2018-03-14 | GSE102249 | GEO

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