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Differentially expressed genes between YY1 knockdown and control cells, transfected in colorectal cancer cell line DLD-1

ABSTRACT: Differentially expressed genes between YY1 knockdown and control cells, transfected in colorectal cancer cell line DLD-1

PROVIDER: PRJNA798456 | ENA |

REPOSITORIES: ENA

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Differentially expressed genes between YY1 knockdown and control cells, transfected in colorectal cancer cell line DLD-1

Project description:Yin Yang 1 (YY1) is a multifunctional transcription factor with critical roles in carcinogenesis and metastasis. However, its biological role and clinical impact in colorectal cancer (CRC) remain unclear. This study aimed to elucidate the oncological role of YY1 in CRC. To indentify the target genes of YY1, microarray analysis was performed on siYY1 and siControl transfected cells.

2022-01-19 | GSE193948 | GEO

Differentially expressed genes between YY1 knockdown and control cells, transfected in colorectal cancer cell line SW48

2022-01-19 | GSE193949 | GEO

Differentially expressed genes between YY1 knockdown and control cells, transfected in colorectal cancer cell line SW48

Project description:Differentially expressed genes between YY1 knockdown and control cells, transfected in colorectal cancer cell line SW48

| PRJNA798455 | ENA

Expression data from in vitro polarized-pathogenic Th17 system transfected with YY1 shRNA lentivirus

Project description:The proportion of pathogenic Th17 (pTh17) cells were significantly higher in RA than that in control individuals and showed a potential relevance with YY1 expression. Increased YY1 expression was observed in pTh17, and the pTh17 differentiation was hampered by YY1 knockdown. We used microarrays to detail the gene expression changes of pTh17 transfected with YY1 shRNA lentivirus and tried to elucidate the potential mechanisms how YY1 can affect pTh17 cell differentiation in RA.

2021-09-30 | GSE184979 | GEO

HMGA2-driven gene expression in human colorectal DLD-1 cells.

Project description:Gene expression analysis of stable HMGA2-overexpressing DLD-1 cells (DLD-1-HMGA2) and its control cells (DLD-1-Vector).

2019-08-29 | GSE136544 | GEO

miR-21-5p-driven gene expression in human colorectal DLD-1 cells.

Project description:Gene expression analysis of stable miR-21-5p-overexpressing DLD-1 cells (DLD-1-miR-21) and its control cells (DLD-1-miR-Vec).

2019-08-31 | GSE136665 | GEO

ChIP-seq of AP4 binding in human colorectal cancer cells DLD-1.

Project description:In order to comprehensively identify genes directly regulated by AP4, a genome-wide chromatin-immunoprecipitation analysis (ChIP) followed by next generation sequencing (ChIP-seq) was performed after activation of a conditional AP4 allele in DLD-1 cells. One DLD-1 Sample was sequenced.

2013-05-31 | E-GEOD-46935 | biostudies-arrayexpress

RNA-seq in HepG2 cells with YY1 knockdown

Project description:Dysregulation of YY1 has been observed in many human cancers. Recent studies have shown that overexpression of YY1 plays a pivotal role in the initiation and progression of nonalcoholic fatty liver disease (NAFLD) which may further progress into hepatocellular carcinoma (HCC). To obtain a detailed view of genes and pathways regulated by YY1 in the liver, we carried out global RNA-seq in liver HepG2 cells with YY1 knocked down.

2021-06-04 | GSE158884 | GEO

Assessing the dynamics and macromolecular interactions of the intrinsically disordered protein YY1

Project description:YY1 is a ubiquitously expressed, intrinsically disordered transcription factor involved in neural development. The oligomeric state of YY1 varies depending on the environment. These changes may alter its DNA binding ability and hence its transcriptional activity. In addition to its oligomeric state, the interaction of YY1 with proteins such as FOXP2 can impact its role in transcription. The aim of this work is to study the structure and dynamics of YY1 binding to DNA and to determine the influence of oligomerisation and associations with FOXP2 on its DNA binding mechanism. Size exclusion chromatography, fluorescence anisotropy and electrophoretic mobility shift assays were used to study YY1 oligomerisation and interaction with FOXP2. To better understand potential structural changes to YY1 upon DNA binding, hydrogen deuterium exchange mass spectrometry was used. The results indicate that YY1 consists of specific structured regions, while most of the sequence remains disordered. Furthermore, the oligomeric nature of the protein is dependent on ionic strength. DNA affects oligomerisation and the protein undergoes changes in structure and dynamics upon DNA binding. YY1 and FOXP2 were found to interact with each other both in isolation and in the presence of YY1-specific DNA. The heterogeneous, dynamic multimerisation of YY1 identified in this work is, therefore, likely to be important for its ability to make heterologous associations with other proteins such as FOXP2. The interactions that YY1 forms with itself, FOXP2 and DNA form part of an intricate mechanism of transcriptional regulation by YY1, which is vital for appropriate neural development.

2024-01-26 | PXD038431 | Pride

Expression changes in Yy1 knock down mouse embryonic stem cells

Project description:We have determined the global gene expression upon loss of function of the Yy1 transcription factor in mouse embryonic stem cells Total RNA was extracted from ES cells transiently transfected with yy1-specific or scrampled-control siRNA oligos 48 hr post-transfection.

2012-01-04 | E-GEOD-31784 | biostudies-arrayexpress

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