Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell RNA-seq of Wild Type and Cd226-/- Thymus Transcriptomes

ABSTRACT: Single-cell RNA-seq of Wild Type and Cd226-/- Thymus Transcriptomes

PROVIDER: PRJNA800037 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Single-cell RNA-seq of Wild Type and Cd226-/- Thymus Transcriptomes

Project description:Thymi from WT and Cd226 strain knockout mice were digested to obtain single-cell suspensions and then loaded onto microfluidic devices for scRNA-seq. The scRNA-seq libraries were constructed and sequencing outputs were demultiplexed using the bcl2fastq to convert Binary Base Call files into FASTQ format.

2022-01-27 | GSE194270 | GEO

Analysis of Wild Type and MFN2-/- Macrophage Transcriptomes

Project description:Analysis of Wild Type and MFN2-/- Macrophage Transcriptomes

| PRJNA715471 | ENA

Single cell RNA-seq of metabolite specific T-cells (MR1-restricted) from adult mouse thymus

Project description:Mucosal-Associated Invariant T cells (MAIT cells) have a unique specificity for the microbial metabolite 5-OP-RU presented by the non-classical presentation molecule MR1. Upon activation, they release cytotoxic mediators and engage an antimicrobial activity. As a subset of T lymphocytes, MAIT development occurs in the thymus where they acquire their effector phenotype under the control of the key transcription factor ZBTB16. This particular maturation process is in contrast with conventional T cells that egress the thymus with a naive phenotype before populating the secondary lymphoid organs, and the molecular events driving the MAIT lineage decision are poorly known. In the present work, we evaluated the transcriptional events and the role of the slam-SAP pathway on the lineage decision of MR1-restricted T cells by single cell RNAseq. MAIT cells undergoing positive selection were FACS-sorted with a MR1:5-OP-RU labeled tetramer, from thymus of wild-type and sapKO mice. Their transcriptomes were captured using a 10x chromium system.

2019-07-01 | E-MTAB-7704 | biostudies-arrayexpress

Analysis of Wild Type and Whsc1-/- small intestine Transcriptomes

Project description:Analysis of Wild Type and Whsc1-/- small intestine Transcriptomes

| PRJNA743911 | ENA

RNA-seq analysis of Wild Type and Mll5-/- Retinal Transcriptomes

Project description:Analysis of differential gene expression in wild type vs Mll5-/- Retinal Transcriptomes. The aim is to identify potential genes that are regulated by MLL5.

2022-02-20 | GSE196935 | GEO

Single cell RNA sequencing of mouse donor T cells after graft-versus-host disease (GVHD) model

Project description:Lethally irradiated mice were transferred with bone marrow cells together with allogeneic Treg cells isolated from CD226+/+ TIGIT+/+, CD226-/- TIGIT+/+, CD226+/+ TIGIT-/-, or CD226-/- TIGIT-/- mice, followed by allogeneic wild-type Tconv cells two days later to induce acute GVHD. We performed scRNA sequencing of donor T cells isolated from the spleen of recipient mice eight days after GVHD induction.

2021-05-18 | GSE155250 | GEO

RNA-seq analysis of the stem of wild type and γVPE transcriptomes

Project description:RNA-seq was performed to examine the differential expressed transcriptomes in γVPE plants during stem development compare to wild type plants.

2019-02-16 | GSE126624 | GEO

RNA-seq Analysis of the stem of Wild Type and HDT1 Transcriptomes

Project description:RNA-seq was performed to examine the differential expressed transcriptomes in hdt1 plants during stem development compare to wild type plants.

2018-05-31 | GSE115066 | GEO

RNA-seq Analysis of the stem of Wild Type and CEP1 Transcriptomes

Project description:RNA-seq was performed to examine the differential expressed transcriptomes in cep1 plants during stem development compare to wild type plants.

2017-08-16 | GSE102694 | GEO

Next Generation Sequencing of Wild Type and Oslec1-1 Transcriptomes

Project description:Next Generation Sequencing of Wild Type and Oslec1-1 Transcriptomes

| PRJNA745207 | ENA