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RNA-seq from MFN2;Stra8-Cre cKO vs WT spermatocytes in mice

ABSTRACT: RNA-seq from MFN2;Stra8-Cre cKO vs WT spermatocytes in mice

PROVIDER: PRJNA800395 | ENA |

REPOSITORIES: ENA

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RNA-seq from MFN2;Stra8-Cre cKO vs WT spermatocytes in mice

Project description:The Mfn2 gene was conditionally knock-out from male mice germ cells using Stra8-Cre mice. The spermatocytes were collected from PD 24 and PD 52 male mice, and were further isolated as pachytene cells (P) and leptotene/zygotene cells (L/Z). The transcriptome between MFN2 WT and MFN2 cKO spermatocytes were analyzed via RNA-seq in P and L/Z populations at different ages, PD 24 amd PD 52, respectively.

2022-05-04 | GSE194377 | GEO

Gene expression in pachytene spermatocytes and round spermatids depleted of HDAC3

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Single-cell RNA-sequencing of intratibial LLC cancer model in DMP1-Cre Mfn2-/- mice, DMP1-Cre Rhot1-/- mice, and wild-type mice.

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RNAseq analysis of Chrnb4-cre driven Dicer conditional knockout (Dicer CKO) whole retinas

Project description:We have shown that Chrnb4-cre; Dicerflox/flox retinas (Dicer CKO) display a cone dystrophy by postnatal day P21, independent of rod degeneration. To elucidate which gene pathways were affected by Dicer loss, RNA sequencing was performed in whole neural retinas of P21 control and Dicer CKO mice. This experiment aims to identify which genes were differentially expressed between Dicer conditional knockout and control mice.

2019-01-14 | E-MTAB-6133 | biostudies-arrayexpress

miRNAseq analysis of Chrnb4-cre driven Dicer conditional knockout (Dicer CKO) whole retinas

Project description:We have shown that Chrnb4-cre; Dicerflox/flox retinas (Dicer CKO) display a cone dystrophy by postnatal day P21, independent of rod degeneration. To elucidate which miRNAs were involved in such phenotype, miRNA sequencing was performed in whole retinas of P21 and 3.5 month old control and Dicer CKO mice. This experiment aims to identify which miRNAs were differentially expressed in Dicer conditional knockout mice when compared to control mice.

2019-01-14 | E-MTAB-6132 | biostudies-arrayexpress

Next Generation Sequencing of Loxp (Rxratm1Krc/J,Rxra loxP), Hetero(Nex-cre;Rxratm1Krc/J,Rxra cKO-Het) and cKO (Nex-cre;Rxratm1Krc/J,Rxra cKO) mouse Transcriptomes

Project description:Next Generation Sequencing of Loxp (Rxratm1Krc/J,Rxra loxP), Hetero(Nex-cre;Rxratm1Krc/J,Rxra cKO-Het) and cKO (Nex-cre;Rxratm1Krc/J,Rxra cKO) mouse Transcriptomes

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Project description:Mus musculus strain:Stra8-cre Raw sequence reads

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Project description:Mus musculus strain:Stra8-cre Raw sequence reads

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Gene expression profiling of Smad1/5 cKO and Acvr2a cKO mice

Project description:We generated mice with single or double conditional inactivation of SMAD1 and SMAD5 using progesterone receptor (PR) cre (Smad1flox/flox;Smad5flox/flox;Pgr-cre+/-, or “Smad1/5 cKO”). Female mice with single SMAD1 or SMAD5 deletion were subfertile, whereas Smad1/5 cKO were infertile and had no visible implantation sites at 4.5 days post-coitum (dpc), indicating functional redundancy of SMAD1 and SMAD5. Histological and molecular analyses of the Smad1/5 cKO uteri during pregnancy determined that the infertility was the result of impaired uterine receptivity. During the window of implantation, uteri of Smad1/5 cKO mice responded abnormally to estradiol (E2) and to progesterone (P4), retained luminal PR expression, and displayed cytoplasmic FOXO1 mis-localization. Furthermore, uteri of Smad1/5 cKO mice did not respond to an artificial decidual stimulus and the stroma failed to differentiate. To determine the cell surface receptor complex that controls BMP signaling during implantation, we generated mice with conditional deletion of Acvr2a and Acvr2b using Pgr-cre+/-. We determined that Acvr2b cKO females were subfertile, while Acvr2a cKOs were infertile and displayed a range of ovarian and uterine abnormalities, including endometrial and implantation defects that phenocopied those of Smad1/5 cKO mice. Transcriptomic profiling of the Smad1/5 cKO and Acvr2a cKO uterus showed that genes involved in epithelial cell remodeling and microvilli/ciliated cell function were overrepresented in both genotypes. These results demonstrate that BMP signals mediated via ACVR2A and SMAD1/5 control endometrial receptivity and embryo implantation by remodeling the apicobasal polarity of the epithelium during the window of implantation.

2021-04-23 | GSE152675 | GEO

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Project description:We have generated CRISPR edited versions of hESC line MShef11 to produce MFN2 R94Q/+ and MFN2 R94Q/R94Q lines as a model for Charcot Marie Tooth Disease (CMT) 2A. This were differentiated to limb innervating motor neurons, the predominantly affected cell time in CMT2A and RNA was examined to investigate differences in cell lines.

2022-07-28 | E-MTAB-10831 | biostudies-arrayexpress

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