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Identification of a LncRNA EPR—>METTL7A1 pathway modulating translation of select genes (Total RNA)

ABSTRACT: Identification of a LncRNA EPR—>METTL7A1 pathway modulating translation of select genes (Total RNA)

PROVIDER: PRJNA802226 | ENA |

REPOSITORIES: ENA

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Identification of a LncRNA EPR—>METTL7A1 pathway modulating translation of select genes (Total RNA)

Project description:EPR is a long non-coding RNA (lncRNA) that controls cell proliferation in mammary gland cells by regulating gene transcription and, here, we report on Mettl7a1 as a target of EPR. We show that the lncRNA induces Mettl7a1 transcription by remodeling the 3-dimensional chromatin structure at the Mettl7a1 locus. Our data indicate that METTL7A1 participates in the EPR-dependent pathway that antagonizes TGF-β signaling. METTL7A1 is absent in tumorigenic murine mammary gland cells and its human ortholog (METT7A) is downregulated in breast cancers. Importantly, expression of METTL7A1 in 4T1 tumorigenic cells reduces their transformation potential, and the putative methyltransferase activity of METTL7A1 appears dispensable for its biological functions. METTL7A1 is a cytoplasmic protein and, from a mechanistic perspective, interacts with factors implicated in the early steps of mRNA translation, associates with ribosomes, and affects the levels of select proteins without substantial changes in mRNA abundance. Our data suggest the possibility that METTL7A1 conveys the transcriptional regulation operated by EPR into specific changes of mRNA translation.

2022-05-28 | GSE195822 | GEO

Identification of a LncRNA EPR—>METTL7A1 pathway modulating translation of select genes (Polysomes)

2022-05-28 | GSE195821 | GEO

Identification of a LncRNA EPR—>METTL7A1 pathway modulating translation of select genes (3C-Seq)

2022-05-28 | GSE195819 | GEO

Identification of a LncRNA EPR—>METTL7A1 pathway modulating translation of select genes

Project description:Identification of a LncRNA EPR—>METTL7A1 pathway modulating translation of select genes

| PRJNA802224 | ENA

Identification of a LncRNA EPR—>METTL7A1 pathway modulating translation of select genes (Polysomes)

Project description:Identification of a LncRNA EPR—>METTL7A1 pathway modulating translation of select genes (Polysomes)

| PRJNA802228 | ENA

Identification of a LncRNA EPR—>METTL7A1 pathway modulating translation of select genes (3C-Seq)

Project description:Identification of a LncRNA EPR—>METTL7A1 pathway modulating translation of select genes (3C-Seq)

| PRJNA802229 | ENA

LncRNA EPR-induced METTL7A1 modulates target gene translation

Project description:EPR is a long non-coding RNA (lncRNA) that controls cell proliferation in mammary gland cells by regulating gene transcription. Here, we report on Mettl7a1 as a direct target of EPR. We show that EPR induces Mettl7a1 transcription by rewiring three-dimensional chromatin interactions at the Mettl7a1 locus. Our data indicate that METTL7A1 contributes to EPR-dependent inhibition of TGF-β signaling. METTL7A1 is absent in tumorigenic murine mammary gland cells and its human ortholog (METTL7A) is downregulated in breast cancers. Importantly, re-expression of METTL7A1 in 4T1 tumorigenic cells attenuates their transformation potential, with the putative methyltransferase activity of METTL7A1 being dispensable for its biological functions. We found that METTL7A1 localizes in the cytoplasm whereby it interacts with factors implicated in the early steps of mRNA translation, associates with ribosomes, and affects the levels of target proteins without altering mRNA abundance. Overall, our data indicates that METTL7A1 —a transcriptional target of EPR— modulates translation of select transcripts.

2022-08-12 | PXD034398 | Pride

Identification of a LncRNA EPR—>METTL7A1 pathway modulating translation of select genes

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-05-28 | GSE195823 | GEO

LncRNA EPR transcriptional activity controls intestinal mucus and prevents susceptibility to inflammation and tumorigenesis [RNA-seq]

Project description:The lncRNA EPR (a.k.a BC030870) is highly enriched in the gastrointestinal tract in mice and, more specifically, in the large intestine. We generated conditional EPR knock-out in large intestine e and analyzed genome-wide the gene expression changes by RNA-Seq. Total RNA was extracted from the proximal colon of six control (EPR fl/fl) and six knock-out (EPR cKO) mice. Libraries were constructed and sequenced. Results indicate that EPR knock-out predominantly leads to down-regulation of genes implicated in mucus biogenesis. These results help in explaining the phenotype displayed by EPR cKO mice that is characterized by higher susceptibility to intestinal inflammation and cancer formation.

2023-03-28 | GSE218994 | GEO

LncRNA EPR transcriptional activity controls intestinal mucus and prevents susceptibility to inflammation and tumorigenesis SW480 [RNA-Seq]

2023-03-28 | GSE219181 | GEO

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