Project description:The objectives of the present study were to analyze the changes on miRNA expression profile in swine spleen using next-generation sequencing (NGS) technology and bioinformatic analysis at 10, 25 and 50 days post inoculation (DPI) with Chinese I genotype strain of T. gondii. Compared to control group, 34, 6 and 86 differentially expressed miRNAs (DEMs) were respectively found in spleens of infected pigs at 10, 25 and 50 DPI. The results not only showed that the miRNA expression of the host can be changed by the invasion of T. gondii, but also revealed the differences on the regulation of the key biological processes or pathways involved in host responses between the acute and chronic T. gondii infection.
Project description:RNA sequencing and ATAC-seq of conventional NK cells and ILC1s in uninfected mice and mice that were infected 5 wk previously with the Type II Prugniaud strain of T. gondii
Project description:The spleen is a site of acute infection following challenge with the parasite Toxoplasma gondii. We utilized scRNA sequencing to analyze the immune response to this infection.
Project description:Purpose: Porcine alveolar macrophage was infected by T. gondii including Rh strain and Me49 strain. We want to explore the change of miRNAs after infected with T. gondii in porcine alveolar macrophages. Results: Our study generated six mi RNA expression profiles from macrophages which infect with Rh strain and Me49 and control group in different time. Compare with T. gondii-infected and uninfected with T. gondii, 81 differentially expressed mi RNAs were identified, including 36 novel mi RNAs and 45 mature mi RNAs.
Project description:Through RNA sequencing and reactome analyses, we report that type I interferon response is elicited in the spleen of HIV-1-infected humanized mice when compared to mock-infected humanized mice.
Project description:Through RNA sequencing and gene ontology analyses, we report that immune activation is elicited in the spleen of 4 HIV-1-infected humanized mice when compared to 4 mock-infected humanized mice.
Project description:Toxoplasma gondii (T. gondii) is an obligate intracellular parasite that can infect almost all warm-blooded animals, causing serious public health problems. Lysine crotonylation (Kcr) is a newly discovered posttranslational modification (PTM), which has been proved that is relevant to procreation regulation, active transcription and cell signaling pathway. However, the biological functions of crotonylation have not yet been reported in macrophages infected with T. gondii. In our study, we performed a ChIP-seq analysis of porcine alveolar macrophages infected with T. gondii RH to explore the relationship of histone Kcr with T. gondii infection.
Project description:Toxoplasma gondii is an obligate intracellular parasite causing severe diseases in immunocompromised individuals and congenitally infected neonates, such as encephalitis and chorioretinitis. This study aimed to determine whether serum metabolic profiling can (i) identify metabolites associated with oocyst-induced T. gondii infection and (ii) detect systemic metabolic differences between T. gondii-infected mice and controls. We performed the first global metabolomics analysis of mice serum challenged with 100 sporulated T. gondii Pru oocysts (Genotype II). Sera from acutely infected mice (11 days post-infection, dpi), chronically infected mice (33 dpi) and control mice were collected and analyzed using LC-MS/MS platform. Following False Discovery Rate filtering, we identified 3871 and 2825 ions in ESI+ or ESI- mode, respectively. Principal Component Analysis (PCA) and Partial Least Squares Discriminant Analysis (PLS-DA) identified metabolomic profiles that clearly differentiated T. gondii-infected and -uninfected serum samples. Acute infection significantly influenced the serum metabolome. Our results identified common and uniquely perturbed metabolites and pathways. Acutely infected mice showed perturbations in metabolites associated with glycerophospholipid metabolism, biosynthesis of amino acid, and tyrosine metabolism. These findings demonstrated that acute T. gondii infection induces a global perturbation of mice serum metabolome, providing new insights into the mechanisms underlying systemic metabolic changes during early stage of T. gondii infection.
2018-04-16 | MTBLS418 | MetaboLights
Project description:Epididymis of mice infected with Toxoplasma gondii
Project description:Understanding altered expression of proteins and transcripts associated with Toxoplasma gondii infection in cats may improve our understanding of how this parasite manipulates the molecular microenvironment of the definitive host. We performed proteomics analysis of six organs (brain, heart, spleen, liver, lung and small intestine) in cats acutely infected with T. gondii. A total of 32,657 proteins were identified among the six examined organs, including 2,556 differentially expressed proteins (DEPs), of which 1,325 DEPs were up-regulated and 1,231 DEPs were down-regulated. The brain, liver, lung, spleen, heart and small intestine exhibited 125 DEPs, 463 DEPs, 255 DEPs, 283 DEPs, 855 DEPs and 675 DEPs, respectively. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were performed on all proteins and DEPs in all organs, showed that many proteins were enriched in binding, cell part, cell growth and death, signal transduction, translation, sorting and degradation and immune system. Correlation between proteins and transcripts with differential expression patterns were detected in the heart (n = 9), liver (n = 19), lung (n = 9), small intestine (n = 17), and spleen (n = 3). These DEPs were mainly involved in immune response, tryptophan catabolism, and extracellular matrix remodeling. Future investigations are needed to identify the pathophysiological mechanisms underlying the reported associations between the identified proteins and transcripts and T. gondii infection.
