Project description:Double-digest Restriction-site associated DNA sequencing (ddRAD-seq) of wild mouse lemurs (genus Microcebus)
| PRJNA318921 | ENA
Project description:Double digest restriction associated DNA (ddRAD) sequencing of 108 samples from the R. arundanum group
| PRJNA554975 | ENA
Project description:Population genetic structure of Spodoptera exigua in China using double-digest restriction site-associated DNA sequencing
| PRJNA649670 | ENA
Project description:Restriction-site associated DNA
| PRJNA588306 | ENA
Project description:Restriction site-associated DNA sequencing
| PRJNA766857 | ENA
Project description:Genome-wide SNPs reveal fine-scale population structure of Laodelphax striatellus in China using double-digest restriction site-associated DNA sequencing
Project description:We analyzed levels of 5-methyl cytosine nnnn CCCGGG target sites by sequential restriction digest by SmaI and XmaI enzymes, ligating Illumina adaptors to the restriction fragments and reading methylation-specific signatures at the ends of restriction fragments by paired ends Illumina high throughput sequencing. Digital restriction enzyme analysis of methylation (DREAM) was performed to determine the methylation profile of SW48 colon cancer cell line genomic DNA. Genomic DNA spiked in with unmethylated, partially methylated and fully methylated standards was sequentially cut at CCCGGG sites with the methylation-sensitive enzyme SmaI (blunt ends) and its methylation-tolerant neoschizomer XmaI (5'CCGG overhangs), creating different end sequences that represented methylation status of the CCCGGG sites. These end sequences were analyzed by Illumina high throughput sequencing. Methylation status at individual CCCGGG sites across the genome was determined by counting the methylated reads with the CCGGG signature and unmethylated reads with the GGG signature at the beginnings of the sequencing reads after alignment to the human genome.