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Targeted gene expression profiling of human myeloid cells from blood and lung compartments of patients with Tuberculosis and other lung diseases

ABSTRACT: Targeted gene expression profiling of human myeloid cells from blood and lung compartments of patients with Tuberculosis and other lung diseases

PROVIDER: PRJNA808999 | ENA |

REPOSITORIES: ENA

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Targeted gene expression profiling of human myeloid cells from blood and lung compartments of patients with Tuberculosis and other lung diseases

Project description:This was a pilot study aimed at uncovering the role of myeloid-derived suppressor cells (myeloid-derived suppressor cell) in patients with Tuberculosis and differences between cells found in peripheral blood and in the lung. Fluidigm™ RT-PCR technology was used to quantify expression levels of 43 genes in monocytecytes and myeloid-derived suppressor cell from peripheral blood and monocytecytes and alveolar macrophages from bronchoalveolar lavage fluid taken from patients with Tuberculosis or other lung diseases.

2022-02-22 | GSE197113 | GEO

Human Ex Vivo Lung Perfusion: A Model System to study Lung diseases and direct targeted therapies

Project description:In the present study, we demonstrate the feasibility of the EVLP technique to perfuse lungs from deceased patients with a broad spectrum of chronic lung diseases. We were able to assess gene expression changes over the course of EVLP and between end-stage diseases by RNA sequencing transcriptomics from tissue samples collected at different time points, revealing fundamental genetic information which has important avenues for modulation in the future. Thus, EVLP can serve as a clinically relevant experimental model to derive mechanistic insights into pulmonary pathophysiology and various human lung diseases.

2021-01-21 | GSE146436 | GEO

Human Ex Vivo Lung Perfusion: A Model System to study Lung diseases and direct targeted therapies

Project description:Human Ex Vivo Lung Perfusion: A Model System to study Lung diseases and direct targeted therapies

| PRJNA610580 | ENA

Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single-cell profiling

Project description:Similar to the brain, the eye is considered an immune-privileged organ where tissue-resident macrophages provide the major immune cell constituents. However, little is known about spatially restricted macrophage subsets within different eye compartments with regard to their origin, function and fate during health and disease. Here, we combined single-cell analysis, fate mapping, parabiosis and computational modelling to comprehensively examine myeloid subsets in distinct parts of the eye during homeostasis. This approach allowed us to identify myeloid subsets displaying diverse transcriptional states. During choroidal neovascularization, a typical hallmark of neovascular age-related macular degeneration (AMD), we recognized disease-specific macrophage subpopulations with distinct molecular signatures. Our results highlight the heterogeneity of myeloid subsets and their dynamics in the eye. This provides new insights into the innate immune system in this organ and may offer new therapeutic targets for ophthalmological diseases.

2021-01-19 | GSE160797 | GEO

miRNA Profiles in lung cancer patients and controls with other non-cancer lung diseases

Project description:This project analyzes plasma profiles of patients suffering from lung cancer and controls with other non-cancer lung diseases Analysis of plasma obtained from 26 patients collected at up to 8 time points and from 12 controls

2015-09-24 | E-GEOD-68951 | biostudies-arrayexpress

Myeloid cell RelA/p65 promotes lung cancer proliferation

Project description:Smoking is the most important risk factor for both lung cancer (LC) and chronic obstructive pulmonary disease (COPD). The aim of this study was to investigate the role of myeloid cell NF-kB in the regulation of tumor cell growth signaling. We subjected mice lacking myeloid RelA/p65 to a metastatic LC model. Cigarette smoke (CS) exposure significantly increased the proliferation of Lewis lung carcinoma cell (LLC) tumors in wild type mice. In CS exposed mice lacking myeloid RelA/p65, the tumor growth was largely inhibited. Transcriptome and pathway analysis of cancer tissue revealed a fundamental impact of myeloid cells on various growth signaling pathways. Myeloid RelA/p65 is necessary to link smoke-induced inflammation with LC growth. Keywords: Expression profiling by array Analysis of gene expression in lewis lung carcinoma cells resected from lungs of WT and RelA/p65 deficient mice exposed to smoke or air. Four different samples were analyzed (3 replicates each).

2012-03-31 | E-GEOD-36568 | biostudies-arrayexpress

miRNA Profiles in lung cancer patients and controls with other non-cancer lung diseases

Project description:This project analyzes plasma profiles of patients suffering from lung cancer and controls with other non-cancer lung diseases

2015-09-24 | GSE68951 | GEO

Supersulphide airway protection in viral and chronic lung diseases

Project description:Supersulphides (inorganic and organic sulphides with sulphur catenation) manifest diverse physiological functions. These supersulphides are mainly generated from mitochondrial cysteinyl-tRNA synthetase (CARS2) that functions as a principal cysteine persulphide synthase (CPERS). Here we found powerful protective functions of supersulphides in viral airway infections (influenza and COVID-19) and chronic lung diseases, including chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis (IPF), and aged lungs. Enhanced supersulphide exhalation also occurred from human airways in COVID-19 as well as the hamster model of SARS-CoV-2 infection, as identified by breath supersulphur-omics that we developed herein. The lung damage related to oxidative stress and inflammation in mouse models of COPD, IPF, and ageing and the lethal effects that resulted were mitigated by endogenous supersulphides, supplied via CARS2/CPERS or exogenously by the supersulphide donor glutathione trisulphide. Our findings thus elucidated the airway protective role of supersulphides and their therapeutic potential in viral and chronic lung diseases.

2023-07-14 | PXD043607 | Pride

Small RNA Sequencing of Different Blood Compartments

Project description:Small RNA Sequencing of Different Blood Compartments

| PRJNA823739 | ENA

Genome-wide analysis of lung myeloid dendritic cells gene expression from healthy or emphysema subjects

Project description:Lung myeloid dendritic cells isolated from emphysema but not healthy subjects induce Th1 and Th17 cells differentiation. The goal of this study is to identify the genes differentially expressed by lung myeloid dendritic cells from healthy or emphysema subjects. These genes may play crucial roles in directing Th1 and Th17 cells differentiation. Total mRNA was extracted from lung myeloid dendritic cells isolated from lungs of three healthy controls and three emphysema patients.

2011-01-01 | E-GEOD-26296 | biostudies-arrayexpress

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