Project description:We used single cell RNA-seq to comprehensively map raphidophyte Heterosigma akashiwo to grow under present and future ocean conditions. We unveiled several unique types of metabolic changes, signaling pathways, and regulons associated with these cells.
Project description:<p>Four species of phytoplankton representing important bloom-forming species from three globally important phyla (Bacillariophyta, Haptophyta, and Ochrophyte) were cultured in this study. These species include the cosmopolitan diatom <em>Chaetoceros affinis</em> CCMP159 (isolated from Great South Bay, NY, USA, 1958), the haptophytes<em> Chrysochromulina polylepis </em>CCMP1757 (isolated from the North Sea 1988) and <em>Gephyrocapsa oceanica</em> RCC1303 (isolated from Arachon Bay, France, Jan 1999), and the raphidophyte <em>Heterosigma akashiwo </em>strain CCMP 2393 (isolated from Rehoboth Bay, Delaware, USA). Cultures were grown under three conditions: nitrogen-stress, phosphorus-stress, and replete conditions. Intracellular metabolites were extracted from cultures and analyzed with targeted and untargeted mass spectrometry-based metabolomics methods.</p>
Project description:we applied a metaproteomic approach to investigate the global protein expression profiles of two co-existing phytoplankton species H. akashiwo and P. donghaiense during their bloom periods, and characterized the differentially expressed proteins. Our results indicated different light-harvesting ability and nutritional niche divergence in utilization of carbon, nitrogen and phosphorus drove bloom occurrence of the two phytoplankton species under different ambient conditions.
