Project description:HSPD1 knockdown was established in A549 cells using an shRNA approach. Secretomes from both shHSPD1-A549 and shControl-A549 cells were collected and subjected to label-free comparative proteome analysis using SWATH-MS.
Project description:We identified a tumor-supressing long non-coding RNA (lncRNA) named lncRNA induced by TGF-beta and antagonizes TGF-beta signaling 1 (LITATS1). It was found to mitigate epithelial-to-mesenchymal transition (EMT), cell migration and extravasation in a zebrafish exnograft model. To better dissect the downstream target genes and elucidate the signaling pathways altered by LITATS1, we depleted LITATS1 by specific shRNAs in A549 cells. Total RNA was collected and sent for RNA sequencing analysis. Afterwards, differentially expressd genes were used for the pathway enrichment analysis and gene set enrichment analysis (GSEA).
Project description:We depleted TIMM13 using short interfering RNA (siRNA) in A549 lung cancer cells, and found that silencing of TIMM13 could significantly inhibit A549 cell proliferation. Thus, we conducted RNA-seq in the A549 cells transduced with either nontargeting siRNA or two distinct TIMM13-specific siRNAs. We observed widespread gene expression change in A549 cells upon TIMM13 knockdown.