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Differential gene expression upon ADAR3 expression in U87 glioblastoma cells

ABSTRACT: Differential gene expression upon ADAR3 expression in U87 glioblastoma cells

PROVIDER: PRJNA815860 | ENA |

REPOSITORIES: ENA

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Differential gene expression upon ADAR3 expression in U87 glioblastoma cells

Project description:The genes that are differentially expressed in ADAR3 expressing cells compared to control U87 cells were identified.

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Identification of ADAR3-bound targets in U87 cells

Project description:We report transcripts bound by ADAR3 through RNA immunoprecipitation of U87 cells expressing 3X-FLAG tagged ADAR3 compard to control cells

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Transcriptome-wide analysis of RNA editing changes in response to ADAR3 expression in U87 cells

Project description:We report RNA editing changes across the trancriptome in U87 cells that express ADAR3 compared to control cells.

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Project description:Role of ADAR3 in U87 cells

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Project description:We have analyzed RNA-seq data to identify A-to-I editing sites in two groups of samples: one group isolated from human U87 cell line expressing an active ADAR3 mutant while the other isolated from U87 cell line expressing the inactive counterpart of the ADAR3 mutant. We compared these two groups of samples and identified sites whose editing levels are higher in the first group than in the second group.

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Identification of ADAR3-bound targets in U87 cells

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Identifying molecular targets of endogenous ADAR3 in gliolastoma cells

Project description:Through immunoprecipitation of epitope-tagged ADAR3 protein, several ADAR3-bound RNAs have been reported till date. However, exogenous expression of proteins are usually associated with altered gene expression profiles and thus, can influence binding profiles of RNA-binding proteins (RBPs) being studied. Here, we used affinity-purified antibody to pull down ADAR3 endogenously expressed in U373 glioblastoma cells and identified transcripts significantly enriched in the ADAR3 immunoprecipitants over control. To serve as control, antisera isolated from rabbit prior to immunization with ADAR3 antigen was used to perform the IP.

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Transcription profiling array of human glioblastoma cell line U87 comparing control cells with U87-shLIN7a knockdown cells and rescue cells which have the same Lin7a knockdown but re-express Lin7a lentivirally (U87-shLin7a+Lin7A)

Project description:After performing an in-vivo screening with U87 glioblastoma cells transduced with a knockdown library several genes could be identified. Lin7a which was one of the candidates was further evaluated. Single knockdown of Lin7a in U87 conferred a pro-invasive phenotype in-vitro and in-vivo. Overexpression of Lin7a in the Primary glioblastoma cell line T269 reduced its invasive phenotype. To decipher the underlying pathways U87 control, U87-shLIN7a and U87-shLin7a+Lin7A (rescue cells after re-expression of Lin7A) were analyzed after in-vitro culture by a transcription profiling Array.

2017-08-27 | E-MTAB-5871 | biostudies-arrayexpress

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