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Bronchoalveolar lavage alveolar macrophage subset transcriptomes in WT and NOX2-knockout mice

ABSTRACT: Bronchoalveolar lavage alveolar macrophage subset transcriptomes in WT and NOX2-knockout mice

PROVIDER: PRJNA816851 | ENA |

REPOSITORIES: ENA

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Bronchoalveolar lavage alveolar macrophage subset transcriptomes in WT and NOX2-knockout mice

Project description:The leukocyte NADPH oxidase 2 (NOX2) plays a key role in pathogen killing and immunoregulation. Genetic defects in NOX2 result in chronic granulomatous disease (CGD), associated with microbial infections and inflammatory disorders, often involving the lung. Alveolar macrophages (AM) are the predominant immune cell in the airways at steady state, and limiting their activation is important given constant exposure to inhaled materials, yet the importance of NOX2 in this process is not well-understood. Here, we show a previously undescribed role for NOX2 in maintaining lung homeostasis by suppressing AM activation, as studied using CGD mice or mice with selective loss of NOX2 primarily in macrophages. AM lacking NOX2 have increased cytokine responses to TLR2 and TLR4 stimulation ex vivo. Moreover, between 4 and 12 weeks of age, mice with global NOX2 deletion developed an activated CD11bhigh subset of AM with epigenetic and transcriptional profiles reflecting immune activation compared to WT AM. The presence of CD11bhigh AM in CGD mice correlated with increased numbers of alveolar neutrophils and proinflammatory cytokines at steady state as well as increased lung inflammation following insults. Moreover, deletion of NOX2 primarily in macrophages was sufficient for mice to develop an activated CD11bhigh AM subset and accompanying pro-inflammatory sequela. Additionally, we showed that the altered resident macrophage transcriptional profile in the absence of NOX2 is tissue-specific as these changes were not seen in resident peritoneal macrophages. Thus, these data demonstrate that absence of NOX2 in alveolar macrophages leads to their pro-inflammatory remodeling and dysregulates alveolar homeostasis.

2022-04-03 | GSE198769 | GEO

bronchoalveolar lavage fluid

Project description:bronchoalveolar lavage fluid Metagenome

| PRJNA449183 | ENA

DNA methylation profiles of cells commonly found in bronchoalveolar lavage

Project description:Genome wide DNA methylation profiling of 4 cell populations purified from paediatic bronchoalveolar lavage samples. The cell types profiled are alveolar macrophages, granulocytes, lymphocytes and alveolar epithelial cells. Fluorescence-activated single cell sorting was used to purify the cell populations of interest using previously described methods (Shanthikumar, AJRCMB, 2020 ;63(2):152-159). Genome wide DNA methylation profiling was performed via the EPICArray. These data can be used for reference based deconvolution of cell proportion of bronchoalveolar lavage samples. DNA extracted from raw BAL (n=6) was also profiled using the EPICArray, allowing for validation of reference based deconvulution.

2021-10-09 | GSE185556 | GEO

Human Airway and Alveolar Organoids from Bronchoalveolar Lavage Fluid

Project description:Many lung diseases remain understudied due to a lack of experimental models. Lung organoids, which consist of self-organizing epithelial cells, provide versatile in vitro models for normal and abnormal biology, drug screening, gene editing, and personalized therapeutics. However, human organoids are generally derived from lung tissue, which is not commonly obtained and represents only a small fraction of lung pathologies. Induced pluripotent stem cells have provided an important alternative but require complex manipulation. Recently, one study reported airway organoids from bronchoalveolar lavage (BAL) fluid, though sample sizes and characterization were limited. Here, we demonstrate robust establishment of airway organoids from a variety of human BAL samples and show that these organoids consist predominantly of basal cells plus differentiated airway cell types including secretory, ciliated, KRT13+ “hillock,” and ionocyte cells. Furthermore, we report the development of BAL-derived alveolar organoids comprised of alveolar type 2 (AT2) cells. These techniques significantly expand the scope of lung diseases that can be studied using safely accessible primary human cells.

2024-05-01 | GSE254166 | GEO

Clinical bronchoalveolar lavage (BAL) samples

Project description:Clinical bronchoalveolar lavage (BAL) samples Targeted loci

| PRJNA408304 | ENA

Bronchoalveolar lavage of healthy human adults

Project description:Bronchoalveolar lavage of healthy human adults

| PRJNA637757 | ENA

Workflow for metaproteome characterization in bronchoalveolar lavage fluid (BALF)

Project description:Establish a pipeline for identifying bacterial peptides from human bronchoalveolar lavage fluid samples.

2019-06-24 | PXD008273 | Pride

Proteomic analysis of bronchoalveolar lavage fluid in COVID-19 patients

Project description:Proteomic analysis of bronchoalveolar lavage fluid in COVID-19 patients

2020-10-20 | PXD022085 | iProX

Transcription profiling of bronchoalveolar lavage cells from AJ mice treated with urethane vs. controls

Project description:AJ mouse is susceptible to lung carcinogenesis from urethane treatment and is a good model for human adenocarcinoma. We completed a study using microarray analysis of bronchoalveolar lavage cells from control or urethane treated mice. A unique macrophage expression signature in the lung tumor microenvironment was able to correctly classify the lavage samples. Experiment Overall Design: RNA from bronchoalveolar lavage cells of age matched untreated AJ mice controls (C) or from urethane treated (T) AJ mice was prepared. Datasets were accurately classified using a unique macrophage gene expression signature derived from the tumor microenvironment.

2008-04-07 | E-GEOD-7258 | biostudies-arrayexpress

Cigarette smoking alters mRNA expression in human alveolar macrophages

Project description:Alveolar macrophages from never smokers and active smokers were isolated by bronchoalveolar lavage and gene expression was measured. Chronic cigarette smoke exposure, as occurs in smoker's lungs, leads to significant changes in gene expression. Of note, RNA was isolated immediately following bronchoscopy. Alveolar macrophage levels were >95%. 4 never smokers and 4 active smokers were recruited for the study. After bronchoalveolar lavage and RNA isolation, gene expression was measured using Affymetrix Human Exon 1.0 ST arrays.

2012-10-02 | E-GEOD-34517 | biostudies-arrayexpress

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