Project description:There are 10 mice in the experiment, named REC. The mice were fed with high salt diets (5% NaCl) for 4 weeks and then fed with normal salt diets for 4 weeks. Then extracted DNA from mice gastric flora to detect changes in the gastric flora of mice.
Project description:Divided into four groups,CTL,Salt,(S+B),B.CTL group was fed with normal salt diets for 4 weeks,Salt group were fed with high salt diets(5% NaCl)for 4 weeks,(S+B)group were fed with high salt diets mixed with buckwheat (1:1)for 4 weeks ,B group: first fed high salt diets for 4 weeks and then fed with buckwheat for 4 weeks. Extract mouse gastric flora DNA and sequence.
Project description:Although microbiota plays a critical role for the normal development and function of host immune systems, the detail of the influence, especially on those in the large intestine (LI), remains unknown. Here, we performed transcriptome analysis using Affymetrix GeneChip on LI of germ-free (GF) and specific pathogen free (SPF) mice of IQI, an inbred strain established from ICR. Keywords: comparison of the mice with or without enteric flora