Sulfate-reducing bacteria consortium SRB1
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ABSTRACT:
PROVIDER: PRJNA820631 | ENA |
REPOSITORIES: ENA
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Project description:a sulfate-reducing bacteria consortium
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Project description:Copper (Cu) is an essential micronutrient required as a co-factor in the catalytic center of many enzymes in bacteria. However, excess Cu is hazardous and can generate pleiotropic effects. Cu has been the metal of choice for piping used in household water distribution systems. Due to its leaching from pipelines, Cu is present at an elevated concentration in groundwater and in soil which is of public health concern. Sulfate-reducing bacteria (SRB) have been demonstrated to remove toxic levels of Cu. However, reports on the toxicity of Cu towards SRB have primarily focused on the degree of toxicity and subsequent elimination. In this study, we show in detail the Cu(II) stress-related effects on a model sulfate reducing bacteria, Desulfovibrio alaskensis G20. Cu(II) stress effects were assessed as alterations in the transcriptome through RNA-Seq at varying Cu(II) concentrations (5µM and 15µM). In the pairwise comparison of control vs 5µM, 61.43% of genes were downregulated and 38.57% genes were upregulated. In 15µM vs control, 49.51% genes were downregulated, and 50.5% genes were upregulated. The results indicated that the expression of inorganic ion transporters and translation machinery was massively modulated. Moreover, changes in important biological processes such as DNA transcription and signal transduction were observed at high Cu(II) concentration. In addition, metabolomics analysis indicated the effect of certain organic acids and amino acids in cellular metal buffering system and reducing oxidative damage to cells. These results will help us better understand the mechanism of Cu(II) stress response and provide avenues for future research.
2022-05-31 | GSE191076 | GEO
Project description:Iron-reducing bacteria 1 Raw sequence reads
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Project description:sulfate-reducing enrichment culture Genome sequencing and assembly
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Project description:Sulfate-reducing bacteria (SRB) colonize the guts of ~50% of humans. We used genome-wide transposon mutagenesis and insertion-site sequencing (INSeq), RNA-Seq, plus mass spectrometry to characterize genetic and environmental factors that impact the niche of Desulfovibrio piger, the most common SRB in a surveyed cohort of healthy USA adults. Gnotobiotic mice were colonized with an assemblage of sequenced human gut bacterial species with or without D. piger and fed diets with different levels and types of carbohydrates and sulfur sources. Diet was a major determinant of functions expressed by this artificial 9-member community and of the genes that impact D. piger fitness; the latter includes high- and low-affinity systems for utilizing ammonia, a limiting resource for D. piger in mice consuming a polysaccharide-rich diet. While genes involved in hydrogen consumption and sulfate reduction are necessary for its colonization, varying dietary free sulfate levels did not significantly alter levels of D. piger, which can obtain sulfate from the host in part via cross-feeding mediated by Bacteroides-encoded sulfatases. Chondroitin sulfate, a common dietary supplement, increased D. piger and H2S levels without compromising gut barrier integrity. A chondroitin sulfate-supplemented diet together with D. piger impacted the assemblage’s substrate utilization preferences, allowing consumption of more reduced carbon sources, and increasing the abundance of the H2-producing Actinobacterium, Collinsella aerofaciens. Our findings provide genetic and metabolic details of how this H2-consuming SRB shapes the responses of a microbiota to diet ingredients, and a framework for examining how individuals lacking D. piger differ from those that harbor it. 8 samples total, 2 gropus of 4 mice: Proximal colon gene expression profiles of gnotobiotic mice colonized with an artificial gut community composed of 8 human gut species (group 1: NoDp) and from mice colonized with the same community plus D. piger (Dp). Mice were fed a HF/HS diet supplemented with 3% chondroitin sulfate. Animals were sacrificed 2 weeks after colonization
2013-07-12 | E-GEOD-48808 | biostudies-arrayexpress