Project description:wild-type and safa-/- THP-1 cells, or Flag-SAFA and Flag-Del-RGG stable expressed SAFA-/- cells were infected with VSV for 24 hours, samples were collected and send for RNA-seq.
Project description:Genome wide RNA-Seq screen was did to detect gene expression of HSV-1 and Hela . We constructed a HeLa cell line that stably expresses the plasmids of SAFA or SAFA-ADAR1 catalytic domain(SAFA-ADAR1cd) or SAFA-ADAR1 catalytic domain E488Q(SAFA-ADAR1cd E488Q) . The RNAs were harvested followed by RNA sequencing to identify the edited RNA.
Project description:Flag-SAFA or Flag-Del-RGG mutant stablely expressed THP-1 cells were infected with VSV for 6 hours, samples were collected and send for ATAC-seq.
