Project description:This experiment was used to determine the effect of a botanical insecticide upon gene expression profiles in Drosophila melanogaster. Adult female Drosophila (oregon-R strain) were treated with an ethylacetate extract of Piper nigrum (Piperaceae) seeds formulated in 99% ethanol. Treatment was topical, using a Potter's tower to administer a total of 2 mL of a 0.9mg/mL concentration. Control treatment was identical except flies were treated with 99% ethanol as a solvent control. Gene expression was studied four hours post-treatment. Keywords: insecticide response, stress-response
Project description:This study was designed to evaluate the proteome profiles of Piper nigrum when challenged with three different biotic stress. We have utilized a transcriptome-assisted protein identification pipeline coupled with a BLAST2GO annotation.
