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Gene expression comparison between yeast histone H2B WT and H2B R95A mutant without and with rapamycin treatment.

ABSTRACT: Gene expression comparison between yeast histone H2B WT and H2B R95A mutant without and with rapamycin treatment.

PROVIDER: PRJNA831653 | ENA |

REPOSITORIES: ENA

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Gene expression comparison between yeast histone H2B WT and H2B R95A mutant without and with rapamycin treatment.

Project description:This microarray data is the Affymetrix array of RNA expressed by Wild Type and Mutant strains yeast that is untreated and treated with rapamycin. In yeast, rapamycin inhibits the TORC1 kinase signaling pathway causing rapid alteration in gene expression and ultimately cell cycle arrest in G1 and this study provides a new insight whereby rapamycin signaling via the Torc1 kinase may exploit the pheromone pathway.

2022-04-25 | GSE201413 | GEO

methylation sites of Histone H2B catalyzed by LaeA

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Project description:The study aims to elucidate the effect of mono-ubiquitination of histone H2B at K123.

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Histone H2B^3-32, H2B K->G, H2B^3-37, and H2B^30-37 mutations

Project description:Histone H2B^3-32, H2B K->G, H2B^3-37, and H2B^30-37 mutations

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Genomic expression comparison between WT and H2B(K123R)

Project description:The study aims to elucidate the effect of mono-ubiquitination of histone H2B at K123. The WT and H2B(K123R) yeast in early stationary phase were inoculated in fresh YPD rich media at OD 0.3. The cells were then grown to OD2.0 and their total RNA was isolated for genomic expression profiling with S98 arrays (Affymetrix). The experiments were performed in triplicate.

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2011-10-27 | GSE33050 | GEO

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Project description:Histone H2B was mutated to give H2B^3-32, H2B K->G, H2B^3-37, and H2B^30-37. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B HBR domain. This SuperSeries is composed of the SubSeries listed below.

2006-04-08 | GSE3806 | GEO

GlcNAcylation of histone H2B facilitates its monoubiquitination [Affymetrix data]

Project description:We have found that histone H2B is GlcNAcylated at residue S112 by O-GlcNAc transferase and that H2B S112 GlcNAcylation fluctuates in response to extracellular glucose level. We have also found that H2B S112 GlcAcylation promotes H2B K120 ubiquitination. To investigate whether these histone modification correlate to transcriptional activation, we performed comprehensive gene expression analysis using Affymetrix GeneChip in HeLa cell cultured with different conditions, i.e. without glucose, with glucose and with FBS. HeLa cells were cultured in DMEM with the following three conditions, 1) DMEM without glucose for 24 hours, 2) DMEM without glucose for 24 hours and followed by treatment with 4.5 g/L glucose for another 24 hours, 3) normal culture condition (DMEM with FBS). Total RNA was purified from these cells and each RNA was linearly amplified and hybridized to Affymetrix GeneChip.

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Project description:Although ADP-ribosylation of histones by PARP-1 has been linked to genotoxic stress responses, its role in physiological processes has remained elusive. We found that ADPribosylation of histone H2B-Glu35 inhibits the differentiation of adipocyte precursors, leading to a reduction of newly formed adipocytes in white adipose tissue. ADP-ribosylation of Glu35 by PARP-1 inhibits phosphorylation of adjacent H2B-Ser36, which is required for the proadipogenic gene expression program. It also inhibits adipogenesis in cultured cells and a mouse lineage tracing genetic model. The activity of PARP-1 on H2B requires NMNAT-1, a nuclear NAD+ synthase, which serves as a specificity factor to direct PARP-1 catalytic activity to Glu and Asp residues. Collectively, our results demonstrate a functional interplay between H2B-Glu35 ADP-ribosylation and H2B-Ser36 phosphorylation that controls adipogenesis and cancer cell proliferation.

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