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MRNAseq of murine qPSCs after culture with conditioned media

ABSTRACT: mRNAseq of murine qPSCs after culture with conditioned media

PROVIDER: PRJNA835819 | ENA |

REPOSITORIES: ENA

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Murine blastocysts release mature microRNAs into culture media that reflect developmental status

Project description:Extracellular microRNA sequences have attracted interest for their potential use as accessible biomarkers that may be non-invasively collected from a wide range of biological fluids, including spent culture media. The ongoing milieu between the late-stage preimplantation embryo and the receptive uterus remains an actively investigated area of research and the physiological role of secreted microRNA sequences remains to be determined. Spent culture media used to culture late stage E4.0 murine blastocysts was screened for 641 mature microRNA sequences using a qPCR-based array. We report here the 39 sequences that were exclusively detected in the conditioned media using this approach. The embryonic miR-290 family were among the most abundant extracellular sequences. Notably, implantation-relevant members miR-126a, miR-101a, miR-143 and miR-320 were also detected.

2021-01-16 | GSE138285 | GEO

mRNAseq of murine qPSCs after culture with conditioned media

Project description:we examined the influence of Tc17-CM from WT or from IL17A/F KO mice on the in vitro differentiation of quiescent pancreatic stellate cells (qPSC) towards an iCAF or myCAF phenotypes

2022-05-09 | GSE202377 | GEO

In-depth proteome analysis of Hela cell culture conditioned media containing fetal bovine serum.

Project description:We performed DIA-MS based proteomic analysis of Hela cells conditioned media containing fetal bovine serum by albumin (Alb)-depletion and non-depletion. In addition, we attempted to enrich secreted proteins by reducing the volume of culture medium (10mL, 5mL and 2mL). The supernatants of each culture condition were subjected to albumin depletion followed by DIA-MS. We further analyzed proteins in the conditioned media of HeLa cells with and without tumor necrosis factor (TNF) stimulation.

2022-02-22 | PXD024272 | JPOST Repository

NRVM Conditioned Media Proteomics

Project description:Proteomic results from NRVM conditioned media overexpressing GRK2

2024-04-22 | PXD050356 | Pride

RNA-Sequencing of Astrocytes Stimulated with Activated Endothelial conditioned media (ECM) and Activated Microglia conditioned media (MCM)

Project description:The goal of this study is to compare the mRNA transcriptome of astrocytes treated with Activated Microglia and Endothelial conditioned media

2022-03-18 | GSE175485 | GEO

Wildtype and Gde3 knockout conditioned media from primary astrocyte culture

Project description:Primary astrocyte cultures were prepared from wildtype and Gde3 knockout mouse brain. Conditioned media was collected, concentrated and analyzed using TMT-MS.

2023-03-20 | MSV000091515 | MassIVE

mRNAseq of murine PancOVA tumor cells after coculture with Tc17 conditioned -iCafs or Tc17 cells

Project description:we examined the influence of Tc17 conditioned -iCafs or Tc17 cells on the in vitro differentiation of murine PancOVA tumor cells

2023-02-10 | GSE218816 | GEO

Analysis of conditioned media cytokine levels of breast cancer cell lines

Project description:To identify the cytokines secreted by mesenchymal-like cancer cells that activate macrophages, the cytokine profiles of conditioned media from MCF7, MCF7 induced to undergo EMT by treatment of TGF-β, TNF-α and prolonged mammosphere culture, and MDA-MB-231 cells were analyzed by RayBio® Human Cytokine Antibody Array V.

2013-11-01 | GSE51938 | GEO

Proteomics analysis of murine lung organoids

Project description:Using proteomics technique to analysis molecular lanscape of murine lung organoid in different culture media

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RNA-seq of murine macrophages (J774A.1 and Raw264.7) grown for 48 hours in conditioned media collected from primary murine Triple Negative Breast Cancer cells

Project description:The goal of the experiment was to demonstrate if the overexpression of human-Prune-1 in Triple Negative breast cancer cells induces M2-polarization of macrophages in vitro. For this purpose, murine primary cells from breast tumor developed by Genetically Engineered Mouse Models (GEMMs) of TNBC (i.e., MMTV-Wnt1) and metastatic TNBC overexpressing both human Prune-1 and Wnt1 in mammary gland (i.e., MMTV-Prune-1/Wnt1) were obtained. Conditioned media were collected from these primary cells (1x106 cells) after 24 hours. Murine macrophages (J774A.1 and Raw264.7; 1x106) were starved for six hours and then grown for 48 hours in those conditioned media collected from MMTV-Wnt1 and MMTV-Prune-1/Wnt1 cells. Untreated macrophages were used as negative control for the experiment.

2020-12-03 | E-MTAB-9231 | biostudies-arrayexpress

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