Redefining electrical stimulation safety using spatial transcriptomics
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ABSTRACT:
PROVIDER: PRJNA835900 | ENA |
REPOSITORIES: ENA
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Project description:Current standards for safe delivery of electrical stimulation to the central nervous system are based on foundational studies which examined post-mortem tissue for histological signs of damage. This set of observations and the subsequently proposed limits to safe stimulation, termed the “Shannon limits,” allow for a simple calculation (using charge per phase and charge density) to determine the intensity of electrical stimulation that can be delivered safely to brain tissue. In the three decades since the Shannon limits were reported, advances in molecular biology have allowed for more nuanced and detailed approaches to be used to expand current understanding of the physiological effects of stimulation. Here, we investigated spatial transcriptomics as a new approach to assess the safety and efficacy of electrical stimulation in the brain. Electrical stimulation was delivered to the rat visual cortex with either acute or chronic electrode implantation procedures (acute: tissue collection 3 hours post-stimulation on the day of surgery; chronic: stimulation delivered 1-month post-implantation, and tissue collection 24 hours later). To explore the influence of device type and stimulation parameters, we used carbon fiber ultramicroelectrode arrays (7 µm diameter) and microwire electrode arrays (50 µm diameter) delivering charge and charge density levels selected above and below reported tissue damage thresholds (range: 2-20 nC, 0.1-1 mC/cm2). Spatial transcriptomics was performed using Visium Spatial Gene Expression Slides (10x Genomics), which enabled simultaneous immunohistochemistry and spatial transcriptomics to directly compare traditional histological metrics to transcriptional profiles within each tissue sample. Our data revealed unique spatial patterns of differentially expressed genes that are related to cellular processes including inflammation, cell cycle progression, and plasticity. Effects were dependent on stimulation parameters and were localized to both traditional and ultra-small device locations. The abundance of data gathered using this approach allows for sophisticated analysis that can be used to generate new hypotheses while also revealing novel potential biomarkers of neurostimulation.
2022-07-01 | GSE202425 | GEO
Project description:Human primary myotubes +/- electrical pulse stimulation
| PRJEB46916 | ENA
Project description:Analysis of the effect of electrical field stimulation frequency at the gene expression level. Electrical stimulation has been shown to mature nascent cardiomyocytes and alter their beating properties. The purpose of the array was to identify potential mediators of these effects. Total RNA was isolated from cardiomyocytes subjected to 0.5 Hz, 1 Hz, or 2 Hz continuous electrical stimulation for 7 days, compared to an unstimulated control. Three samples from each group were analyzed
2015-11-05 | E-GEOD-74669 | biostudies-arrayexpress
Project description:Analysis of the effect of electrical field stimulation frequency at the gene expression level. Electrical stimulation has been shown to mature nascent cardiomyocytes and alter their beating properties. The purpose of the array was to identify potential mediators of these effects.
2015-11-05 | GSE74669 | GEO
Project description:Electrical brain stimulation (EBS) has gained popularity for laboratory and clinical applications. However, comprehensive characterization of the cellular diversity and cell type-specific gene expression changes induced by EBS remains limited, particularly with respect to specific brain regions and stimulation sites. In this study, we present the first single-nucleus RNA sequencing (snRNA-seq) profiles of rat cortex, hippocampus, and thalamus subjected to alternating current electrical stimulation (ACES) at 40 Hz.
2024-03-18 | GSE253672 | GEO
Project description:To investigate the mechanism of electrical stimulation in the repair of spinal cord injury, we established a rat model of spinal cord injury. Then, we used RNA-SEQ data obtained from ES treatment and 6 different rat models of spinal cord injury for gene expression profile analysis.
2023-07-01 | GSE214291 | GEO
Project description:We report the application of RNA sequencing technology for transcriptomics analysis of the effects of electrical stimulation on rat denervated gastrocnemius muscle. Muscle samples were selected for gene expression analyses，corresponding to the following categories: Sham operation rats, (SHAM, n = 3), sciatic nerve injury rats (DN, n = 3), and sciatic nerve injury with electrical stimulation (DN-SM, n = 3). A total of 14,334 genes (DEGs) were detected in this study.
2020-07-01 | GSE117440 | GEO
Project description:Interventions: Transcutaneous electrical acupoint stimulation group :Transcutaneous electrical stimulation was given at the bilateral Hegu, Neiguan, Feet Three Miles, Upper Juxu, Lower Juxu, and Sanyinjiao points 1 day before, during, and 1, 2, and 3 days after surgery, respectively ;False stimulus group :The electrode pads were selected to be attached at the same acupuncture points as in group A, but not energized, i.e., no current stimulation was given to the patients.
Primary outcome(s): Changes in protein-related indexes (total protein, albumin, prealbumin, transferrin) at 1, 3 and 7 days postoperatively
Study Design: Parallel
| 83018 | ecrin-mdr-crc
Project description:Human myogenic progenitor cells (MPCs) were isolated from the gracilis of two patients (1 female and 1 male) undergoing anterior cruciate ligament reconstruction (age 30-34 years) using a human anti-CD56 (1:20, Cat# 355503, Biolegend) antibody for FACS. Cells were passaged 2-3 times in growth media consisting of Ham’s F-10 (Thermofisher), 20% fetal bovine serum (FBS, Atlanta Biological, Minneapolis, MN, USA), 1% penicillin/streptomycin and 10ng/ml basic fibroblast growth-factor (bFGF, Peprotech, Rocky Hill, NJ, USA). Cells were differentiated into myotubes for 5 days using MyoCult differentiation media (Stemcell Technologies, Vancouver, Canada). On the fifth day, fresh media was added just prior to electrical pulse stimulation (E). Cells were then either stimulated at 12 V, 1 Hz, 2 ms for 24 hr (IonOptix C‐Pace EP, Westwood, MA, USA) or had electrodes placed in wells with no stimulation (E(+) or E(-)), followed by immediate RNA extraction using Qiazol (Qiagen, Hilden, Germany).
2021-08-20 | E-MTAB-10833 | biostudies-arrayexpress
Project description:Interventions: Control group:None;TEAS group:Transcutaneous electrical acupoint stimulation (TEAS)
Primary outcome(s): Serum gastrin concentration
Study Design: Parallel
| 2732428 | ecrin-mdr-crc