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Inflammation modulates intercellular adhesion and mechanotransduction in human epidermis via ROCK2

ABSTRACT: Inflammation modulates intercellular adhesion and mechanotransduction in human epidermis via ROCK2

PROVIDER: PRJNA836426 | ENA |

REPOSITORIES: ENA

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Inflammation modulates intercellular adhesion and mechanotransduction in human epidermis via ROCK2

Project description:The cytoskeletal mechanisms regulating inflammatory responses in the epidermis are not well described. Here we addressed this question by inducing a psoriatic phenotype in human keratinocytes and reconstructed human epidermis using a cytokine stimulation model.We report the results from RNA-seq in N/TERT keratinocytes treated with M5 or both M5 and ROCK2 inhibitor KD025.

2022-05-13 | GSE202522 | GEO

HA-tagged ROCK2 plasmid

Project description:HA-tagged ROCK2 plasmid

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Human hepatocellular carcinoma MHCC-97H: Control vs ROCK1 overexpressed vs ROCK2 overexpressed

Project description:Transcriptional profiling of human hepatoellular carcinoma (HCC) cell line: MHCC-97H comparing control untreated MHCC-97H cells with MHCC-97H cells overexpressed ROCK1 protein. Goal was to determine the effect of ROCK1 on global MHCC-97H cell mRNA and lncRNA expression. Transcriptional profiling of human hepatoellular carcinoma (HCC) cell line: MHCC-97H comparing control untreated MHCC-97H cells with MHCC-97H cells overexpressed ROCK2 protein. Goal was to determine the effect of ROCK2 on global MHCC-97H cell mRNA and lncRNA expression. Transcriptional profiling of human hepatoellular carcinoma (HCC) cell line: MHCC-97H comparing MHCC-97H cells overexpressed ROCK1 with MHCC-97H cells overexpressed ROCK2 protein. Goal was to determine the distinction of ROCK1 & 2 on global MHCC-97H cell mRNA and lncRNA expression.

2019-06-19 | GSE122145 | GEO

Serine-threonine Kinase, ROCK2, Regulates Germinal Center B Cell Positioning and Cholesterol Biosynthesis

Project description:Splenic follicular B cells (FoB; B220+GL7-CD38hiCD23+) and Germinal Center B cells (GCB; B220+GL7+CD38lo) were FACS sorted from WT or CD23-Cre.Rock2 mice after 7d post-immunization with NP-CGG. RNA-seq was then employed to compare the transcriptome of GCBs from the indicated mice. Here we show that loss of ROCK2 leads to altered gene expression in GCBs. We also employed ATAC-seq analysis and found that differentially expressed genes from the ROCK2-deficient GC B cells contained loci that were enriched for IRF binding sites.

2020-05-22 | GSE141328 | GEO

Proteomic analysis of integrin adhesion complex-mediated mechanotransduction

Project description:Integrin adhesion complexes were isolated from mouse fibroblasts treated with or without blebbistatin. Adhesion complexes were resolved by SDS-PAGE, digested with trpysin and analysed by LC-MS/MS using an LTQ Velos mass spectrometer

2015-10-19 | PXD000018 | Pride

Selective dysregulation of ROCK2 activity promotes aberrant transcriptional networks in ABC diffuse large B cell lymphoma

Project description:We have found that ROCK2 regulates a transcriptional program in U2932, an ABC-DLBCL cell line by RNA-seq analysis.

2021-06-17 | GSE147521 | GEO

Polyvinyl alcohol/gelatin hydrogels regulate cell adhesion and chromatin accessibility

Project description:Cell adhesion is known to heavily influence cell migration and wound healing. A number of cell adhesion molecules and their roles has been extensively studied. However, the changes of global chromatin accessibility and gene expressions correlated with cell adhesion is still unclear. Here we prepared a series of PVA/Gelatin hydrogels to handily regulate cell adhesion by adjusting gelatin concentration and found that cell adhesion area and the ratio of non-circular cells were increased with the increasement of gelatin concentration while cell circularity was decreased. Our results showed that widespread chromatin accessibility was increased with increasing expression level of histone deacetylase 11 (Hdc11) under poor cell adhesion. Meanwhile, one branch of MAPK pathway - ERK 1/2 which involved in mechanotransduction signaling was also found in the enrichment analysis of downregulated genes. This work provided a handy method to regulate cell adhesion and these findings deepen our understanding of the cell adhesion process and its associated chromatin accessibility.

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Mechanotransduction Map

Project description:PAthway representing mechanotransduction

2023-02-23 | MODEL2302150001 | BioModels

FAK is required for suppressing aberrant nuclear mechanotransduction and DNA methylation to prevent vascular injury

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UBE2A/B is the trans-acting factor mediating mechanotransduction and contact inhibition

Project description:Mechanotransduction converts mechanical stimuli into biochemical signals to regulate cell growth, differentiation, and survival. However, how mechanotransduction is linked to gene expression remains poorly understood. Here, we report the identification of UBE2A as an mechano-sensitive trans-acting factor, which translocates from the cytosol to the nucleus upon mechanical stimuli. UBE2A is an E2 enzyme that catalyzes ubiquitination of histone, which eventually results in transcription activation of downstream genes. Using ChIP-seq and RNAseq analysis, we identify downstream genes of UBE2A, including YAP1, a well-known mechanotransducer. Knock-down of UBE2A/B significantly downregulates YAP1 protein expression. Our findings identify the new mechanotransduction pathway that regulates gene expression through histone ubiquitination by UBE2A/B.

2023-10-11 | GSE214029 | GEO

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