Project description:Glehnia littoralis is a perennial herb growing on the sandy beaches. In this study, high-throughput sequencing of small RNA was performed to identify miRNAs and their response to salt stress in G. littoralis. As a result, 37 conserved miRNAs belonging to 13 families and 37 novel miRNAs were identified. Among the 74 miRNAs, the expression levels of miR-156b*, miR-171, miR-171*, and miR-319 were downregulated and that of miR-399a, miR-399a*, miR-399b, miR-399b*, novel-9 and novel-14* were upregulated under salt stress. Target prediction of salt-responsive miRNAs indicated that these miRNAs exerted a role by regulating specific stress-responsive genes, such as SPLs. However, a lot of target genes of salt-responsive miRNAs were uncharacterized, which implied that there might be some new miRNA-mediated reguatory of salt response in G. littoralis.
2016-07-21 | GSE84621 | GEO
Project description:transcriptome of Glehnia littoralis
Project description:In order to get insights into the ability of ectomycorrhizal fungi to perceive their biotic environment as well as into the mechanisms of the interactions between ectomycorrhizal fungi and soil bacteria, we analysed the transcriptomic response of the ectomycorrhizal fungus L. bicolor and of two beneficial, and neutral soil bacteria during their interactions in vitro.
Project description:Glehnia littoralis is a perennial herb growing on the sandy beaches. In this study, high-throughput sequencing of small RNA was performed to identify miRNAs and their response to salt stress in G. littoralis. As a result, 37 conserved miRNAs belonging to 13 families and 37 novel miRNAs were identified. Among the 74 miRNAs, the expression levels of miR-156b*, miR-171, miR-171*, and miR-319 were downregulated and that of miR-399a, miR-399a*, miR-399b, miR-399b*, novel-9 and novel-14* were upregulated under salt stress. Target prediction of salt-responsive miRNAs indicated that these miRNAs exerted a role by regulating specific stress-responsive genes, such as SPLs. However, a lot of target genes of salt-responsive miRNAs were uncharacterized, which implied that there might be some new miRNA-mediated reguatory of salt response in G. littoralis. Small RNA profiles of G. littoralis under control (CK), salt stress (SS) and (RS)
Project description:In order to get insights into the ability of ectomycorrhizal fungi to perceive their biotic environment as well as into the mechanisms of the interactions between ectomycorrhizal fungi and soil bacteria, we analysed the transcriptomic response of the ectomycorrhizal fungus L. bicolor and of two beneficial, and neutral soil bacteria during their interactions in vitro. We performed nine hybridizations (macroarray) with samples derived from Laccaria bicolor cultivated alone (3 biological replicates), with P. fluorescens BBc6R8 (3 biological replicates) and with Pf29Arp (3 biological replicates)
Project description:In order to get insights into the ability of ectomycorrhizal fungi to perceive their biotic environment as well as into the mechanisms of the interactions between ectomycorrhizal fungi and soil bacteria, we analysed the transcriptomic response of the ectomycorrhizal fungus L. bicolor and the strain Pseudomonas fluorescens Pf29Arp during their interactions in vitro.
