Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA polymerase II and PARP1 shape enhancer-promoter contacts [CUT&Tag]

ABSTRACT: RNA polymerase II and PARP1 shape enhancer-promoter contacts [CUT&Tag]

PROVIDER: PRJNA848807 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

RNA polymerase II and PARP1 shape enhancer-promoter contacts [CUT&Tag]

Project description:What controls enhancer-promoter communication to allow for cis-transcriptional regulation remains a mystery. Here we studied how transcriptional dynamics shapes enhancer-promoter contacts. We demonstrate that enhancer function is reflected in enhancer-promoter contacts frequency which highly depend on active transcription. Pol II pausing, which is widespread across metazoan enhancers and promoters, has a direct effect on focal enhancer-promoter contacts. We confirmed this effect by depleting NELFB, a subunit of the negative elongation factor complex and a pivotal factor in Pol II pausing. Moreover, the catalytic activity of PARP1, a regulator of transcription and chromatin condensation, stabilizes enhancer-promoter contacts globally, but can destabilize contacts by promoting Pol II escape from pausing. Based on these findings, we propose an updated model that couples transcription and enhancer-promoter contacts.

2022-06-18 | GSE206022 | GEO

RNA polymerase II and PARP1 shape enhancer-promoter contacts

Project description:RNA polymerase II and PARP1 shape enhancer-promoter contacts

| PRJNA849263 | ENA

RNA polymerase II and PARP1 shape enhancer-promoter contacts [ChRO-seq]

Project description:RNA polymerase II and PARP1 shape enhancer-promoter contacts [ChRO-seq]

| PRJNA848812 | ENA

RNA polymerase II and PARP1 shape enhancer-promoter contacts [Micro-C]

Project description:RNA polymerase II and PARP1 shape enhancer-promoter contacts [Micro-C]

| PRJNA849260 | ENA

RNA polymerase II and PARP1 shape enhancer-promoter contacts [Micro-C]

2022-06-18 | GSE206131 | GEO

RNA polymerase II and PARP1 shape enhancer-promoter contacts [ChRO-seq]

2022-06-18 | GSE206025 | GEO

Bulk Cut&run and Cut&tag

Project description:Bulk Cut&run and Cut&tag

| PRJNA686390 | ENA

CUT&TAG of OE19 cell line

Project description:To map the chromatin binding sites for various factors in OE19 cells, we performed CUT&TAG.

2023-02-24 | E-MTAB-11356 | biostudies-arrayexpress

HCT116_SMARCA4 CUT&Tag

Project description:To investigate the enrichment of SMARCA4-R1157W mutation on downstream target gene promoters, we performed CUT&Tag experiments in HCT116 cells.

2022-09-04 | GSE212334 | GEO

Cut&Tag of GLI3 binding during early organoid development

Project description:To investigate GLI3 TF targets during organoid fate decisions, we performed Cut&Tag of GLI3 binding

2022-08-28 | E-MTAB-12006 | biostudies-arrayexpress