Project description:What controls enhancer-promoter communication to allow for cis-transcriptional regulation remains a mystery. Here we studied how transcriptional dynamics shapes enhancer-promoter contacts. We demonstrate that enhancer function is reflected in enhancer-promoter contacts frequency which highly depend on active transcription. Pol II pausing, which is widespread across metazoan enhancers and promoters, has a direct effect on focal enhancer-promoter contacts. We confirmed this effect by depleting NELFB, a subunit of the negative elongation factor complex and a pivotal factor in Pol II pausing. Moreover, the catalytic activity of PARP1, a regulator of transcription and chromatin condensation, stabilizes enhancer-promoter contacts globally, but can destabilize contacts by promoting Pol II escape from pausing. Based on these findings, we propose an updated model that couples transcription and enhancer-promoter contacts.

2022-06-18 | GSE206022 | GEO

RNA polymerase II and PARP1 shape enhancer-promoter contacts [Micro-C]

2022-06-18 | GSE206131 | GEO

RNA polymerase II and PARP1 shape enhancer-promoter contacts [ChRO-seq]

2022-06-18 | GSE206025 | GEO

RNA polymerase II and PARP1 shape enhancer-promoter contacts

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-06-18 | GSE206133 | GEO

PRMT6 and PARP1 sequencing in non-treated MDA-MB-231 cells

Project description:Here, we examined the interactions between PRMT6, poly(ADP-ribose) polymerase 1 (PARP1), and the cullin 4 B (CUL4B)-Ring E3 ligase (CRL4B) complex, to form a transcription-repressive complex that co-occupies the core clock gene PER3 promoter.

2023-05-24 | GSE210947 | GEO

Shape of promoter antisense RNAs regulates ligand-induced transcription activation [Shape-Map]

Project description:Shape of promoter antisense RNAs regulates ligand-induced transcription activation [Shape-Map]

| PRJNA686387 | ENA

Dynamic and stable enhancer-promoter contacts regulate terminal differentiation

Project description:Dynamic and stable enhancer-promoter contacts regulate terminal differentiation

| PRJNA330783 | ENA