Project description:Salvia is an important genus from the Lamiaceae with approximately 1000 species distributed globally. Several Salvia species are commercially important because of their medicinal and culinary properties. We report the construction of the first fingerprinting array for Salvia species enriched with polymorphic and divergent DNA sequences and demonstrate the potential of this array for fingerprinting several economically important members of this genus.
Project description:Salvia is an important genus from the Lamiaceae with approximately 1000 species distributed globally. Several Salvia species are commercially important because of their medicinal and culinary properties. We report the construction of the first fingerprinting array for Salvia species enriched with polymorphic and divergent DNA sequences and demonstrate the potential of this array for fingerprinting several economically important members of this genus. In order to generate the Salvia Subtracted Diversity Array (SDA), a Suppression Subtractive Hybridization (SSH) was performed between a pool of ten Salvia species and a pool of non-angiosperm and angiosperms (excluding the Lamiaceae) to selectively isolate Salvia-specific sequences. A total of 285 subtracted genomic DNA (gDNA) fragments were amplified and arrayed. DNA fingerprints were obtained for fifteen Salvia genotypes including three that were not part of the original subtraction pool. Hierarchical cluster analysis indicated that the Salvia-specific SDA was capable of differentiating closely related species of S. officinalis and S. miltiorrhiza and was also able to reveal genetic relationships consistent with geographical origins. Species-specific features were also found for S. elegans, S. officinalis, S. sclarea, S. przewalskii and S. runcinata.
Project description:To identify salvia chinensia benths induced transcriptional changes in triple negative breast cancer cell, RNA-sequencing of MDA-MB-231 cells after salvia chinensia benths treantmnent was performed. Differential gene expression analysis resulted in 7582 differentially expressed genes.
Project description:Dataset used for biochemometric analyses on the extract and fractions of Rosmarinus officinalis (Salvia rosmarinus) tested against MRSA.
Project description:We collected samples of Pistil, Plain Valve, Plain lip and Plain calyx from the same period and quenched them in liquid nitrogen. Two biological replications were performed in each sample. TMT labeled quantitative proteomics was used to analyze different proteins in different tissues. GO and KEGG were used to analyze the differentially expressed proteins in different tissues, and the key proteins in the important pathway were found