Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Craspedostauros australis strain:CCMP3328

ABSTRACT: Craspedostauros australis strain:CCMP3328 Genome sequencing and assembly

PROVIDER: PRJNA850956 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Subversion of host innate immunity by Rickettsia australis via a modified autophagic response in macrophages

Project description:We recently reported that the in vitro and in vivo survival of Rickettsia australis are Atg5-dependent, in association with an inhibited level of anti-rickettsial cytokine, IL-1β. In the present study, we sought to investigate how R. australis interacts with host innate immunity via an Atg5-dependent autophagic response. We found that the serum levels of IFN-γ and G-CSF in R. australis-infected Atg5flox/floxLyz-Cre mice were significantly less compared to Atg5flox/flox mice, accompanied by significantly lower rickettsial loads in tissues with inflammatory cellular infiltrations including neutrophils. R. australis infection differentially regulated a significant number of genes in bone marrow-derived macrophages (BMMs) in an Atg5-depdent fashion as determined by RNA sequencing and Ingenuity Pathway Analysis, including genes in the molecular networks of IL-1 family cytokines and PI3K-Akt-mTOR. The secretion levels of inflammatory cytokines, such as IL-1α, IL-18, TNF-α, and IL-6, by R. australis-infected Atg5flox/floxLyz-Cre BMMs were significantly greater compared to infected Atg5flox/flox BMMs. Interestingly, R. australis significantly increased the levels of phosphorylated mTOR and P70S6K at a time when the autophagic response is induced. Rapamycin treatment nearly abolished the phosphorylated mTOR and P70S6K but did not promote significant autophagic flux during R. australis infection. These results highlight that R. australis modulates an Atg5-dependent autophagic response, which is not sensitive to regulation by mTORC1 signaling in macrophages. Overall, we demonstrate that R. australis counteracts host innate immunity including IL-1β-dependent inflammatory response to support the bacterial survival via an mTORC1-resistant autophagic response in macrophages.

2021-04-06 | GSE171160 | GEO

Elsinoe australis strain:NL1

Project description:Elsinoe australis strain:NL1 Genome sequencing and assembly

| PRJNA384682 | ENA

Photorhabdus australis strain:PB68.1

Project description:Photorhabdus australis strain:PB68.1 Genome sequencing and assembly

| PRJNA296853 | ENA

Glaesserella australis strain:HS4635

Project description:Glaesserella australis strain:HS4635 Genome sequencing and assembly

| PRJNA434506 | ENA

Davidsoniella australis strain:CMW2333

Project description:Davidsoniella australis strain:CMW2333 Genome sequencing and assembly