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The RNA binding protein Swm is critical for Drosophila melanogaster intestinal progenitor cell maintenance

ABSTRACT: The RNA binding protein Swm is critical for Drosophila melanogaster intestinal progenitor cell maintenance

PROVIDER: PRJNA851609 | ENA |

REPOSITORIES: ENA

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The RNA binding protein Swm is critical for Drosophila melanogaster intestinal progenitor cell maintenance

Project description:The regulation of stem cell survival, self-renewal, and differentiation is critical for the maintenance of tissue homeostasis. Although the involvement of signaling pathways and transcriptional control mechanisms in stem cell regulation have been extensively investigated, the role of post-transcriptional control is still poorly understood. Here we show that the nuclear activity of the RNA-binding protein Second Mitotic Wave Missing (Swm) is critical for Drosophila melanogaster intestinal stem cells (ISCs) and their daughter cells, enteroblasts (EBs), to maintain their progenitor cell properties and function. Loss of swm causes ISCs and EBs to stop dividing and instead detach from the basement membrane, resulting in severe progenitor cell loss. swm loss is furthermore characterized by nuclear accumulation of poly(A)+ RNA in progenitor cells. Swm associates with transcripts involved in epithelial cell maintenance and adhesion, and the loss of swm, while not generally affecting the levels of these Swm-bound mRNAs, leads to elevated expression of proteins encoded by some of them, including the fly ortholog of Filamin. Taken together, this study indicates a nuclear role for Swm in adult stem cell maintenance, raising the possibility that nuclear post-transcriptional gene regulation plays vital roles in controlling adult stem cell maintenance and function.

2022-06-25 | GSE206632 | GEO

Lin28-mediated promotion of protein synthesis is critical for neural progenitor cell maintenance and brain development in mice

Project description:Lin28-mediated promotion of protein synthesis is critical for neural progenitor cell maintenance and brain development in mice

| PRJNA543987 | ENA

PRMT1 and CSNK1a1 control epidermal progenitor maintenance

Project description:PRMT1 and CSNK1a1 control epidermal progenitor maintenance

| PRJNA323472 | ENA

LIM domain binding protein 1 regulates a transcriptional program essential for hematopoietic stem cell maintenance

Project description:We used ChIP-Seq to map Ldb1, Scl and Gata2 binding sites in mouse hematopoietic progenitor cells (HPCs). Together with functional studies using conventional and conditional Ldb1 deficient mouse models and bioinformatics analysis, we systematically determined a transcriptional program controlled by Ldb1 complexes in HSC maintenance. To evaluate the role of Ldb1 in hematopoietic stem cell maintenance.

2011-01-04 | E-GEOD-26031 | biostudies-arrayexpress

Gata3 controls stem/progenitor maintenance potential in prostate organoids

Project description:The adult stem/progenitor cell maintenance in the prostate is regulated by a balance between BMP5 self-renewal signal and GATA3 dampening activity. Here we report that the transcription factor GATA3 controls basal stem/progenitor cell maintenance in the prostate by directly controling the expression of BMP5, a key effector of this activity.

2020-08-31 | GSE155289 | GEO

Gata3 controls stem/progenitor maintenance potential in prostate organoids

Project description:Gata3 controls stem/progenitor maintenance potential in prostate organoids

| PRJNA649250 | ENA

LIM domain binding protein 1 regulates a transcriptional program essential for hematopoietic stem cell maintenance

2011-01-04 | GSE26031 | GEO

Lin28-mediated promotion of protein synthesis is critical for neural progenitor cell maintenance and brain development in mice

Project description:Neural progenitor cells (NPCs) undergo rapid proliferation during neurulation in early development. This rapid growth generates a high demand for mRNA translation in a timing-dependent manner, but its underlying mechanism and functional importance remain poorly understood. Lin28 is an RNA-binding protein with two paralogs, Lin28a and Lin28b, in mammals. Lin28b deletion exhibited no developmental defects in mice, while we previously reported that Lin28a deletion led to microcephaly. Here we found that Lin28a/b double knockout (dKO) mice displayed neural tube defects (NTDs) coupled with reduced proliferation and precocious differentiation of NPCs. We used ribosomal protein 24 hypomorphic mice (Rpl24Bst/+) as a genetic tool to dampen global protein synthesis. In support of the importance of Lin28-mediated translation promotion, Lin28a-/-;Rpl24Bst/+ compound mutants exhibited NTDs resembling those seen in Lin28a/b dKO mice. Furthermore, increased NPC numbers and brain sizes in Lin28a-overexpressing mice were rescued by Rpl24Bst/+ heterozygosity. Mechanistically, RNA-sequencing of polysome sucrose gradient fractions revealed a reduced translation of genes involved in the regulation of cell cycle, ribosome biogenesis, and translation in mutants. Lin28a localizes in the nucleoli of NPCs, and ribosome biogenesis was reduced in dKO and increased in Lin28a-overexpressing NPCs. Together, these results suggest that Lin28-mediated promotion of protein synthesis is essential for NPC maintenance and early brain development.

2019-05-22 | GSE131536 | GEO

The protein modifier SUMO is critical for Arabidopsis shoot meristem maintenance at warmer ambient temperatures

Project description:The protein modifier SUMO is critical for Arabidopsis shoot meristem maintenance at warmer ambient temperatures

| PRJNA627419 | ENA

Prionic protein gene dosage is critical for maintenance of neuronal homeostasis of hippocampal circuits

Project description:We demonstrate that Prnp dosage is critical for the maintenance of neuronal homeostasis since both its absence and, more relevantly, its overexpression induce higher sensitivity to kainate (KA) damage. These data correlate with electrophysiological results in freely behaving mutant mice showing an imbalance in activity-dependent synaptic processes, as determined from input/output curves, paired-pulse facilitation, and LTP studies. Gene expression profiling showed that 129 genes involved in canonical pathways such as Ubiquitination or Neurotransmission among others were co-regulated in knockout and PrPc overexpressing mice. RT-qPCR analysis of neurotransmission-related genes confirmed GABA-A and AMPA-Kainate receptor subunit transcriptional co-regulation in both Prnp -/- and Tg20 mice. Our results demonstrate that PrPc is necessary for the proper homeostatic functioning of hippocampal circuits, because of its interactions with GABAA and AMPA-Kainate receptors. Keywords: steady state expression; adult brain tissue; hippocampus; genetic modification; transgenic gain of function mutation; targetted deletion loss of function mutation We performed a global transcriptome analysis of three strains that differ in their Prnp gene dose using Illumina Sentrix 6 mouse v1.1 beadarrays. We analyzed mRNA expression in the hippocampi of four individual male mice from each of three strains: 1) Tg20, with a 30 exogenous copies of the Prnp gene within a Prnp -/- background that overexpresses PrPc about 6-7 times the normal wild type levels; 2) littermates lacking the transgene and therefore carrying only the homozygous knock out Prnp -/- strain which lacks expression of PrPc; and 3) their wild type counterparts carrying two copies of the intact Prnp gene and expressing normal levels of PrPc

2010-04-09 | E-GEOD-16223 | biostudies-arrayexpress

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