Human Oral Keratinocyte Responses to T. denticola infection
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ABSTRACT:
PROVIDER: PRJNA853299 | ENA |
REPOSITORIES: ENA
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Project description:The purpose of this study was to determine, at a global level, the transcriptomic responses of human gingival keratinocytes to T. denticola infection to better understand the disruption of normal barrier immunity that leads to the development of periodontal disease.
2022-06-30 | GSE207003 | GEO
Project description:Individual miRNA analyzed were successfully constructed through nanostring technology of a total of 577 mouse miRNAs in 20 number of SHAM mice and 20 number of T. denticola infected mice, which have been euthanized on the end of 16 weeks infection study.
2023-07-24 | GSE235373 | GEO
Project description:Individual miRNA analyzed were successfully constructed through nanostring technology of a total of 577 mouse miRNAs in 20 number of SHAM mice and 20 number of T. denticola infected mice, which have been euthanized on the end of 16 weeks infection study.
2023-07-24 | GSE235372 | GEO
Project description:Individual miRNA analyzed were successfully constructed through nanostring technology of a total of 577 mouse miRNAs in 20 number of SHAM mice and 20 number of T. denticola infected mice, which have been euthanized on the end of 16 weeks infection study.
2023-07-24 | GSE235371 | GEO
Project description:Individual miRNA analyzed were successfully constructed through nanostring technology of a total of 577 mouse miRNAs in 20 number of SHAM mice and 20 number of T. denticola infected mice, which have been euthanized on the end of 16 weeks infection study.
2023-07-24 | GSE235370 | GEO
Project description:Individual miRNA analyzed were successfully constructed through nanostring technology of a total of 577 mouse miRNAs in 20 number of SHAM mice and 20 number of T. denticola infected mice, which have been euthanized on the end of 16 weeks infection study.
2023-07-24 | GSE235369 | GEO
Project description:Keratinocytes are the major constituent of epithelial cells at mucosal surfaces and skin, which cover organs, internal cavities and the body. Traditionally, keratinocytes have been considered as an inert component of the multilayered epithelium to protect the subepithelial compartments from the pathogenic microorganisms, toxic stimuli and physical trauma. However, accumulated researches of the airway, gastrointestinal tract and skin have demonstrated that keratinocytes function in the development of the immune system, promotion of pathologic inflammation and even impose diverse decisions on immune cells. We used microarrays to detail the global gene expression of oral keratinocyte during oral adaptive immune response. Oral keratinocyte were collected at three time points (control, 48h and 96h) in oral adaptive immune response for RNA extraction and hybridization on Affymetrix microarrays
2011-03-19 | E-GEOD-28035 | biostudies-arrayexpress
Project description:Serum-driven responses, many of which are related to wound healing, are potentially deregulated in cancer development and associated genomic alterations might have prognostic value. The current study assessed fetal bovine serum-induced transcriptomic changes for clinical relevance in head and neck squamous cell carcinoma (HNSCC) using oral keratinocyte models otherwise routinely cultured without serum, including normal keratinocytes (NOK) and the transformed keratinocyte lines SVpgC2a, SqCC/Y1 and LK0412. Bioinformatics-driven analysis of gene expression implicated primarily serum-induced terminal differentiation in NOK including alterations in 99 genes, 13 gene ontology-categories and 6 molecular networks and involvement of 7 key regulator genes. Compared to NOK, the transformed lines expressed around 3-fold lower numbers of differently expressed transcripts, unique sets of gene ontologies, molecular networks and key regulator genes for each line, and consistent absence of terminal differentiation markers. Assessment of the complete in vitro/serum exposure-derived set of differentially expressed genes (totally 180 genes) relative a clinical, information-rich HNSCC data set identified 17 survival-associated genes of which only 12 had previous association to HNSCC. Multi-step validation of the survival-associated genes relative to several independent tumor data sets, including in the Human Gene Expression Map and Human Protein Atlas databases, confirmed novel association to HNSCC for genes COTL1 and INSIG1 and novel poor outcome prediction for the genes CUL4B and PDGFRL. The definition of normal and aberrant serum responses in keratinocyte models therefore coupled new genes to HNSCC including with relevance to prognosis. Analysis of gene expression changes in serum-exposed normal and transformed cells relative the respective un-exposed states. Significantly differentially expressed genes were next assessed by bioinformatics processing using Gene Ontology categories and network analyses. Findings were also validated relative independent HNSCC data sets as well as transcriptomics and proteomics databases.
2014-01-01 | E-GEOD-39376 | biostudies-arrayexpress
Project description:Serum-driven responses, many of which are related to wound healing, are potentially deregulated in cancer development and associated genomic alterations might have prognostic value. The current study assessed fetal bovine serum-induced transcriptomic changes for clinical relevance in head and neck squamous cell carcinoma (HNSCC) using oral keratinocyte models otherwise routinely cultured without serum, including normal keratinocytes (NOK) and the transformed keratinocyte lines SVpgC2a, SqCC/Y1 and LK0412. Bioinformatics-driven analysis of gene expression implicated primarily serum-induced terminal differentiation in NOK including alterations in 99 genes, 13 gene ontology-categories and 6 molecular networks and involvement of 7 key regulator genes. Compared to NOK, the transformed lines expressed around 3-fold lower numbers of differently expressed transcripts, unique sets of gene ontologies, molecular networks and key regulator genes for each line, and consistent absence of terminal differentiation markers. Assessment of the complete in vitro/serum exposure-derived set of differentially expressed genes (totally 180 genes) relative a clinical, information-rich HNSCC data set identified 17 survival-associated genes of which only 12 had previous association to HNSCC. Multi-step validation of the survival-associated genes relative to several independent tumor data sets, including in the Human Gene Expression Map and Human Protein Atlas databases, confirmed novel association to HNSCC for genes COTL1 and INSIG1 and novel poor outcome prediction for the genes CUL4B and PDGFRL. The definition of normal and aberrant serum responses in keratinocyte models therefore coupled new genes to HNSCC including with relevance to prognosis.
2014-01-01 | GSE39376 | GEO
Project description:In our previous study, we identified global genetic and epigenetic aberrations in the tumors of oral squamous cell carcinoma (OSCC) patients who were habitual smokers. We hypothesized that cigarette smoke might play a role in oral malignant transformation. DOK cell line is a dysplasitc oral keratinocyte derived from a heavy smoker with OSCC. The differentially expressed genes between DOK and normal human oral keratinocytes (HOK) may provide important information about OSCC carcinogenesis mediated by cigarette smoking. Total RNA was collected from DOK and HOK cells followed by gene expression microarray analysis.
2015-12-31 | E-GEOD-54861 | biostudies-arrayexpress