Project description:Leaf mold disease caused by Cladosporium fulvum is a major disease in cultivated tomato plants and affects global tomato production. Some Cf genes, of which Cf-16 is an effective gene for resisting tomato leaf mold, are associated with leaf mold resistance; however, the molecular mechanism is largely unknown. We used comparative transcriptome analysis of C. fulvum-resistant (cv. Ontario7816, including the Cf-16 gene) and C. fulvum-susceptible (cv. Moneymaker) tomato lines to identify differentially expressed genes (DEGs) at 4 and 8 days postinfection with C. fulvum. Our results provide new insights into the resistance response mechanism of Cf genes to C. fulvum, especially the unique characteristics of Cf-16 in response to C. fulvum infection.
Project description:Heterologous expression of the fungal pathogen Cladosporium fulvum Avr2 in Arabidopsis plants. Experiment Overall Design: Samples used for microarray analyses were replicated three times in independent experiments and each replication consisted of 10 Avr2-expressing Arabidopsis plants and 10 Col-0 plants grown for four weeks under standard greenhouse conditions
