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Activin A is Required for the Self-Renewal of Aortic Sox2+ Neurospheres

ABSTRACT: Activin A is Required for the Self-Renewal of Aortic Sox2+ Neurospheres

PROVIDER: PRJNA854964 | ENA |

REPOSITORIES: ENA

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Real-Time Quantitative PCR Analyis of Rat Aortic Neurospheres (ANS) treated with Activin A

Project description:Progenitor/stem cell populations of the aorta have been poorly characterized and their self-renewal factors unknown. In this report, we demonstrate that sphere forming progenitor/stem cells, here within termed aortic neurospheres (ANS), can be cultivated from the aorta that are similar to skin-derived precursors (SKPs). Extensive mRNA transcript profiling revealed that ANS abundantly expressed the Inhibin βA transcript, of which the secreted protein dominantly formed the Inhibin βA-βA complex, or Activin A, in the proliferation medium. Co-localization of Activin A, Nestin and Sox2 was observed in the adventitial and peri-adventitial regions of the aorta, indicative of a progenitor/stem cell niche in vivo. Blockade of Activin A signaling, through the neutralization of the Type II Activin receptors ActRIIA and ActRIIB, resulted in the asymmetric differentiation, and epithelial-to-mesenchymal transition of the ANS, respectively. When treated with the latter neutralizing antibody, translocation of the ActRIIB receptor to the nucleus was observed in cells on the periphery of the sphere. Examination of the ActRIIB protein sequence showed the presence of a putative nuclear localization signal of the PY family that was conserved across species. The results from this study demonstrate that Activin A is a self-renewal factor required for maintaining ANS progenitor/stem cell identity and multipotency.

2022-07-05 | GSE207386 | GEO

Activin A is Required for the Self-Renewal of Aortic Sox2+ Neurospheres

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-07-05 | GSE207387 | GEO

Real-Time Quantitative PCR Analyis of Neonatal Rat Aortic Neurospheres (ANS)

2022-07-05 | GSE207380 | GEO

Real-Time Quantitative PCR Analyis of Rat Aortic Neurospheres (ANS) treated with Activin A

Project description:Real-Time Quantitative PCR Analyis of Rat Aortic Neurospheres (ANS) treated with Activin A

| PRJNA854969 | ENA

CUT&RUN Targeting c-FOS, c-JUN and SOX2 in neurospheres derived from mouse brain cells

Project description:Other than in the development of the brain, SOX2 is essential for the long-term self-renewal of neural stem cells (NSCs). The mechanisms of how SOX2 maintains the stemness of NSCs is not yet understood. We have identified Fos as a downstream target of SOX2, and therefore used CUT&RUN to investigate where these transcription factors - and the c-FOS partner c-JUN - interact with the genome. By comparing binding patterns of c-FOS, c-JUN and SOX2, we find that they co-occupate the promoter of the SOCS3 locus, which we also have identified as a gene that rescues SOX2 deletion induced senescence when overexpressed in neurospheres grown from Sox2-deleted mouse NSCs. Taken together, our data provide a basis for elucidating a gene regulatory network necessary for the maintenance of self-renewal in post-embryonic neural stem cells.

2021-04-01 | E-MTAB-9897 | biostudies-arrayexpress

Analysis of SOX2 downregulation under self-renewal conditions.

Project description:SXO2 is one of the factors involved in maintaining self-renewal and pluripotency in hESCs. This study was performed to reveal the effects of reduced SOX2 levels in self-renewing pluripotent hESCs.

2015-11-13 | GSE67993 | GEO

Real-Time Quantitative PCR Analyis of Neonatal Rat Aortic Neurospheres (ANS)

Project description:Real-Time Quantitative PCR Analyis of Neonatal Rat Aortic Neurospheres (ANS)

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Identification the Expression of Sox2 in Colorectal Cancer Tissues

Project description:Sox2 has been studied in several types of human solid tumors. The investigators found that Sox2 had higher expression level in colorectal cancer and metastatic tissues than normal tissues. So the investigators assumed that whether Sox2 plays an important role in the progression and migration of colon cancer.

| 2124687 | ecrin-mdr-crc

Transcription profiling of brain and gut neurospheres of wild type and Bmi-1 knockout mice to reveal that Bmi-1 dependence distinguishes neural stem cell self-renewal from progenitor proliferation.

Project description:The cRNA derived from Brain and Gut neurospheres of wild type and BMI-1 knockout mice were hybridized to Affymetrix mouse ver 2 Chips A, B and C. In case of brain neurospheres, the cRNA were generated by one round RNA amplification (conventional method). In contrast, the cRNA of Gut neurospheres were generated by two round RNA amplifications.

2007-12-15 | E-GEOD-611 | biostudies-arrayexpress

c-Myc Transcriptionally Amplifies Sox2 Target Genes to Regulate Self-Renewal in Multipotent Otic Progenitor Cells [ChIP-Seq]

Project description:An immortalized multipotent otic progenitor (iMOP) cell was generated by transient expression of c-Myc in Sox2-expressing otic progenitor cells. The procedure activated endogenous c-Myc expression in the cells and amplified existing Sox2-dependent transcripts to promote self-renewal. Downregulation of c-Myc expression following growth factor withdrawal resulted in a molecular switch from self-renewal to otic differentiation. ChIP-Seq was accomplished by immunoprecipitating endogenous RNA PolII, c-Myc and Sox2

2015-02-18 | E-GEOD-62513 | biostudies-arrayexpress

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