Project description:Human umbilical vein endothelial cells (HUVEC) were cultured in serum-free medium with 50 μmol/L ADMA (ADMA group) or without ADMA (NA group ). Asymmetric dimethylarginine is a typical uremic toxin which used to induce HUVEC injury.
Project description:Acrolein is a major reactive component of vehicle exhaust, cigarette and wood smoke. It is also present in several food substances and is generated endogenously during inflammation and lipid peroxidation. Although previous studies have shown that dietary or inhalation exposure to acrolein results in endothelial activation, platelet activation and accelerated atherogenesis, the basis for these effects is unknown. Moreover, the effects of acrolein on microRNA (miRNA) have not been studied. Using AGILENT miRNA microarray high throughput technology, we have thus characterized the effects of acrolein exposure on miRNA expression in human umbilical vein endothelial cells (HUVEC).
Project description:We report that miRNA-146a exerts anti-proliferative effects on endothelial cells in vitro as well as in vivo. In order to obtain insights into potential target genes, we generated samples of HUVEC overexpressing miRNA-146a and RNA sequencing was performed by Novogene (Novogene Bioinformatics Technology, Beijing, China)
Project description:The goal of this study is to identify the different proteins secreted by Mycobacterium bovis in the culture media at different stages of bacterial growth. A field strain of M. bovis was allowed to grow in a culture media and culture supernatant was collected at three-time points representing approximately three different phases of growth. The supernatant was digested by trypsin and analyzed by LC-MS/MS analysis
Project description:Shotgun proteomics on sterile culture supernatant from S. aureus clinical isolates to determine proteins associated with pro-inflammatory phenotype.
Project description:We performed miRNA array analysis to analyze the miRNAs secreted into the supernatant during the mesoderm/cardiac differentiation and maturation process from human induced pluripotent stem cells (hiPSCs). Supernatant samples were collected on day -1, day 3, day 5, day 7, day 9, day 21, day 35, and day 51.
Project description:Experiment with 2 conditions: - BAP-PCM : A. glutinosa alone in nitrogen-free culture medium plus frankialess culture medium during 2 days (reference condition). - CN BAP-PCM : A. glutinosa in nitrogen-free culture medium with Frankia culture supernatant during 2 days.
Project description:Acrolein is a major reactive component of vehicle exhaust, cigarette and wood smoke. It is also present in several food substances and is generated endogenously during inflammation and lipid peroxidation. Although previous studies have shown that dietary or inhalation exposure to acrolein results in endothelial activation, platelet activation and accelerated atherogenesis, the basis for these effects is unknown. Moreover, the effects of acrolein on microRNA (miRNA) have not been studied. Using AGILENT miRNA microarray high throughput technology, we have thus characterized the effects of acrolein exposure on miRNA expression in human umbilical vein endothelial cells (HUVEC). 2 condition, acrolein-exposed and vehicle exposed; 4 replicates of each
