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Effect of aging on mitochondrial gene expression using C57BL/6N liver mitochondria

ABSTRACT: Effect of aging on mitochondrial gene expression using C57BL/6N liver mitochondria

PROVIDER: PRJNA858664 | ENA |

REPOSITORIES: ENA

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Effect of aging on mitochondrial gene expression using C57BL/6N liver mitochondria

Project description:To investigate the effect of aging on mitochondrial gene expression we isolated liver mitochondria from 4 12-week and 4 65-week C57BL/6N WT mice. We then isolated RNA and prepared the barcoded library using PCR-cDNA Barcoding Kit SQK-PCB109 and ran it on MinIOn R9.4.1 Flow cells.

2022-07-17 | GSE208177 | GEO

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Project description:ER stress could affect many tissues, especially liver, in which non-alcoholic fatty liver disease, liver steatosis, etc. have been reported relative. But there still lack systematic insight into ER stress in liver, which can be obtained by transcriptomics of the tissue. Here, tunicamycin was utilized to induce ER stress in C57BL/6N mice. And microarray was performed to get the transcriptome alteration. Microarray of liver from tunicamycin-injected C57Bl/6N mice

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Project description:This study aimed at generating a reference data set of C57BL/6N small RNA expression at baseline conditions

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Mus musculus strain:C57Bl/6N

Project description:Mus musculus strain:C57Bl/6N Raw sequence reads

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Gene expression profiling in left cardiac ventricles of C57Bl/6J and C57Bl/6N male mice treated for 48 h with angiotensin II 500 ng/kg/min.

Project description:Analysis of angiotensin II effect on left ventricle at gene expression level. The hypothesis tested in the present study was that angiotensin II treatment may affect gene expression in left ventricle in a strain specific manner. Results provide important information about which genes respond to angiotensin II in C57Bl/6N male mice compared to their C57Bl/6J counterparts. Total RNA obtained from isolated left ventricles from C57Bl/6J and C57Bl/6N mice subjected to 48 hours of angiotensin II infusion, via osmotic mini-pumps (500ng/kg/h) implanted sub-cutaneously, compared to sham operated controls.

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Characterisation of adult C57BL/6N sperm small RNA

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Gene expression profiling in left cardiac ventricles of C57Bl/6J and C57Bl/6N male mice treated for 48 h with angiotensin II 500 ng/kg/min.

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Gene expression in cornea of BALB/c and C57BL/6N mice

Project description:Gene expression was quanitified in 4 naive corneas from BALB/c and 4 corneas from C57BL/6N mice without intervention by RNAseq of total RNA with the Ovation Kit for model organisms. To avoid false positive differential expression from better alignment of the reads from C57BL/6 mice to the reference representing a closely related strain while retaining the applicability of the standard reference genome annotation, two pseudogenomes were generated incorporating the known variants into the reference and aligning to the resulting genomes. BAM files were then converted with Lapels to the standard reference, which includes conversion of genome coordinates and adjusting CIGAR strings. Then expression quantification is possible with respect to the standard gene model (here Ensembl version 94) again.

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Young and Old C57BL/6N Mouse HSC RNA Sequencing

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