Project description:Purpose: The goal of this study is to investigate the role of Brd4 in UV-regulated genome-wide gene expression. Methods: RNA samples from control and Brd4-depleted cells at 0, 0.5 and 2 hr after UV treatment were analyzed using RNA-sequencing (RNA-seq). Results: Signals higher or lower than >1-fold by log2 were regarded as significant and analyzed further. And only either unregulated or downregulated genes in Brd4-depleted cells compared with control cells at the same time point (0, 0.5 or 2 hr) after UV treatment were analyzed. Indeed, a transcriptome analysis indicated that UV-upregulated genes are positively affected by the Brd4 level.
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.