Dataset Information


Homo sapiens

ABSTRACT: Two week Storage and Rehydration of HEK 293 Cells at Ambient Temperature

ORGANISM(S): Homo sapiens  



altmetric image


Long term metabolic arrest and recovery of HEK293 spheroids involves NF-kappaB signaling and sustained JNK activation.

Jack Graham D GD   Garst James F JF   Cabrera M Carla MC   DeSantis Andrea M AM   Slaughter Stephen M SM   Jervis Jody J   Brooks Andrew I AI   Potts Malcolm M   Helm Richard F RF  

Journal of cellular physiology 20060201 2

Understanding how cells withstand a depletion of intracellular water is relevant to the study of longevity, aging, and quiescence because one consequence of air-drying is metabolic arrest. After removal of medium, HEK293 spheroids with intracellular water content of approximately 65% survived partial vacuum, with antistatic control, for weeks in the dark at 25 degrees C. In contrast, only a limited exposure of monolayers to air was lethal; the mitochondrion being a target of this stress. The pat  ...[more]

Similar Datasets

2005-08-01 | GSE1455 | GEO
2008-06-11 | E-GEOD-1364 | ArrayExpress
| PRJNA90119 | ENA
| PRJNA343469 | ENA
2012-03-30 | E-GEOD-28400 | ArrayExpress
2008-06-11 | E-GEOD-1455 | ArrayExpress
| PRJNA258471 | ENA
2004-06-08 | GSE1309 | GEO
2007-08-03 | E-GEOD-1309 | ArrayExpress
2010-01-01 | E-MTAB-122 | ArrayExpress