We have developed a visualization methodology, called a "cluster overlap distribution map" (CODM), for comparing the clustering results of time series gene expression profiles generated under two different conditions. Although various clustering algorithms for gene expression data have been proposed, there are few effective methods to compare clustering results for different conditions. With CODM, the utilization of three-dimensional space and color allows intuitive visualization of changes in c ...[more]
Project description:Inflammation is a key component of pathological angiogenesis. Here we induce cornea neovascularisation using sutures placed into the cornea, and sutures are removed to induce a regression phase. We used whole transcriptome microarray to monitor gene expression profies of several genes Overall design: This was a time course study conprising two arms. In the suture IN arm, sutures were maintained in the cornea after the 0h time point, while suture OUT arm is where both sutures were removed from the cornea at the 0h timpoint. 0h timepoint is when sprouts were 3/4 the distance from the pre-existing limbal vessels towards the sutures. Microarrays were performed at 0h, 24h suture IN and 24h suture OUT. Non sutured eyes from separate animals were used as controls. At each time point except for 0h=3 rats, the rest of the timepoints had four rats each.