Genomics

Dataset Information

235

Saccharomyces cerevisiae


ABSTRACT: TFB5 (YDR079c-a) Knockout Study

ORGANISM(S): Saccharomyces cerevisiae  

PROVIDER: PRJNA90121 | ENA |

REPOSITORIES: ENA

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Publications

Identification of TFB5, a new component of general transcription and DNA repair factor IIH.

Ranish Jeffrey A JA   Hahn Steven S   Lu Yu Y   Yi Eugene C EC   Li Xiao-jun XJ   Eng Jimmy J   Aebersold Ruedi R  

Nature genetics 20040627 7


We previously described the use of quantitative proteomics to study macromolecular complexes. Applying the method to analyze a yeast RNA polymerase II preinitiation complex, we identified a new 8-kDa protein, encoded by the uncharacterized open reading frame YDR079c-a, as a potential new component of the preinitiation complex. Here we show that YDR079c-a is a bona fide component of polymerase II preinitiation complexes and investigate its role in transcription. YDR079c-a is recruited to promoter  ...[more]

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