Project description:Jurkat T cells (in triplicate per treatment group) were left untreated in culture, infected with VSV-G-pseudotyped HIV-based vector (which transduces Tat and eGFP) or treated with TNFalpha. Keywords: parallel sample
Project description:Jurkat T cells (in triplicate per treatment group) were left untreated in culture, infected with VSV-G-pseudotyped HIV-based vector (which transduces Tat and eGFP) or treated with TNFalpha.
Project description:This study examine the transcriptional profiling of HIV dual reporter non-infected, latetnly infected and productively infected Jurkat T cells.
Project description:Jurkat T-cells were latently infected with a defective HIV provirus carrying a GFP reporer gene. The cells were superinfected with a lentiviral vector expressing shRNA to NELF-E. The impact of NELF-depletion on RNAP II distribution on cellular genes and the HIV provirus was measured by ChIP-Seq. Data sets contained between 45 and 56 million mapped reads
Project description:To determine changes of gene expression in human CD4+ T cells (Jurkat) infected with HIV-1 and treated with digoxin. Cells were infected (MOI 0.01) with a single cycle HIV-1 delta env expressing GFP and pseudotyoed with VSV-G in the presence of 400nM digoxin or DMSO. Cells were harvested 36 hours post-infection and processed for RNAseq
Project description:Jurkat T-cells were latently infected with a defective HIV provirus carrying a GFP reporer gene. The cells were superinfected with a lentiviral vector expressing shRNA to NELF-E. The impact of NELF-depletion on RNAP II distribution on cellular genes and the HIV provirus was measured by ChIP-Seq. Data sets contained between 45 and 56 million mapped reads Four samples were compared: Control shRNA, NELF-E shRNA, Control shRNA plus TNF-a, NELF-E shRNA plus TNF-a.
Project description:Time-dependent profile of differentially expressed miRNA following HIV-1 infection. Small RNAs were extracted from HIV-1 infected and non-infected pNL4-3 Jurkat cells at days 0 and 21 and miRNA expression was analyzed by microarray. Keywords: time course
Project description:Time-dependent profile of differentially expressed miRNA following HIV-1 infection. Small RNAs were extracted from HIV-1 infected and non-infected pNL4-3 Jurkat cells at days 0, 7, 21 and 42 and miRNA expression was analyzed by microarray. Keywords: time course
