Project description:In this study, type II alveolar epithelial cells (AEC II) were isolated from normal, hyperoxia exposed (day 0) and recovery (day 1,3,5 and 7) rats with high purity (~95%). Total RNA from isolated cells were used for dual color DNA microarray hybridization with 3DNA 50 kit version 2. Keywords: time-course
Project description:In this study, type II alveolar epithelial cells (AEC II) were isolated from normal, hyperoxia exposed (day 0) and recovery (day 1,3,5 and 7) rats with high purity (~95%). Total RNA from isolated cells were used for dual color DNA microarray hybridization with 3DNA 50 kit version 2. Keywords: time-course
Project description:d-serine is naturally present throughout the human body. It is also used as add-on therapy for treatment-refractory schizophrenia. d-Serine interacts with the strychnine-insensitive glycine binding site of NMDA receptor, and this interaction could lead to potentially toxic activity (i.e., excitotoxicity) in brain tissue. The transcriptomic changes that occur in the brain after d-serine exposure have not been fully explored. Affymetrix microarray technology was used to determine differential gene expression resulting from D-Serine exposure. Keywords: Dose course
