Project description:GFP-labeled touch receptor neurons from C. elegans were dissociated then sorted into Trizol (10,000+ cells per replicate) followed by polyA-enrichment and RNA-seq
Project description:Use of an activated beta-catenin to identify Wnt/beta-catenin pathway target genes in C. elegans, including a subset of collagen genes expressed in late larval development
Project description:Natural genetic variation is the raw material of evolution and influences disease development and progression. To analyze the effect of the genetic background on protein expression in the nematode C. elegans (Caenorhabditis elegans), the two genetically highly divergent wild-type strains N2 (Bristol) and CB4856 (Hawaii) were compared quantitatively. In total, we quantified 3,238 unique proteins in three independent SILAC (stable isotope labeling by amino acids in cell culture) experiments. The differentially expressed proteins were enriched for genes that function in insulin-signaling and stress response pathways.
