Project description:Winter season with reduced day length (photoperiod); led to the growth cessation, dormancy induction and cold acclimation in woody perennial plants. To develop an understanding of the photoperiod signal transduction in Vitis riparia; shoot tip transcriptome profiling was performed under differential photoperiod treatments (long (LD, 15h) and short day (SD, 13h)) for 7 or 21 days after shoots reached 10 nodes (LD7, SD7, LD21 or SD21).
Project description:Drought has become an increasingly important constraint on grapevine sustainability due to global climate change. Vitis riparia, the only grapevine native to the upper Midwest region of the United States, is widely used in scion and rootstock breeding; however, it is not considered drought tolerant. In this study, RNA-Seq data were generated from grapevine root/shoot under WD and well-watered (control (C)) conditions to compare root signaling and shoot responses to water deficit.
Project description:The aim of this quantitative label-free shotgun proteomic experiment is to perform a comparative study of two different sample preparation methods which employ two different pre-fractionation techniques (SDS-PAGE and FASP-GPF) for use in shotgun proteomic analysis of Vitis riparia leaf material with concurrent label-free quantitation in order to optimise a technique that would facilitate identification of most number of proteins and produce useful biological information, when searched against the Vitis vinifera database.
