Genomics,Multiomics

Dataset Information

0

Drosophila melanogaster


ABSTRACT: RNA affinity isolation with TAP-Pum and controls

OTHER RELATED OMICS DATASETS IN: E-GEOD-3581E-GEOD-3580E-GEOD-3582

PROVIDER: PRJNA93669 | ENA |

REPOSITORIES: ENA

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Publications

Genome-wide identification of mRNAs associated with the translational regulator PUMILIO in Drosophila melanogaster.

Gerber André P AP   Luschnig Stefan S   Krasnow Mark A MA   Brown Patrick O PO   Herschlag Daniel D  

Proceedings of the National Academy of Sciences of the United States of America 20060313 12


Genome-wide identification of RNAs associated with RNA-binding proteins is crucial for deciphering posttranscriptional regulatory systems. PUMILIO is a member of the evolutionary conserved Puf-family of RNA-binding proteins that repress gene expression posttranscriptionally. We generated transgenic flies expressing affinity-tagged PUMILIO under the control of an ovary-specific promoter, and we purified PUMILIO from whole adult flies and embryos and analyzed associated mRNAs by using DNA microarr  ...[more]

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