Project description:Mammary gland cells were isolated from 5-8 week old FVB mice and allowed to proliferate as undifferentiated mammospheres in the presence of growth factors (bFGF and EGF). Comparison of the gene expression profiles of undifferentiated mammospheres vs. mammospheres that were allowed to differentiate for 6 days by the removal of growth factors will help to identify genes that are implicated in the maintenance of the mammary gland stem cell compartment. Keywords: other
Project description:Mammary gland cells were isolated from 5-8 week old FVB mice and allowed to proliferate as undifferentiated mammospheres in the presence of growth factors (bFGF and EGF). Comparison of the gene expression profiles of undifferentiated mammospheres vs. mammospheres that were allowed to differentiate for 6 days by the removal of growth factors will help to identify genes that are implicated in the maintenance of the mammary gland stem cell compartment. Experiment Overall Design: this experiment include 2 samples and 12 replicates
Project description:Undifferentiated mammosphere RNA. Samples contain RNA extracted from the cells of mouse mammospheres. Mammosphere are believed to be comprised of stem/progenitor cells (GSM88038). Differentiated mammosphere RNA. RNA extracted from the cells of mouse mammospheres induced to differentiate over a 6-day period. Mammosphere are believed to be comprised of stem/progenitor cells (GSM88039). Keywords: mammosphere differentiation
