Project description:LEAFY COTYLEDON2 activity was induced via a LEC2-Glucocorticoid Receptor fusion protein in Arabidopsis seedlings. Keywords: Ordered
Project description:LEAFY COTYLEDON2 activity was induced via a LEC2-Glucocorticoid Receptor fusion protein in Arabidopsis seedlings. Experiment Overall Design: Transgenic plants carrying a 35S:LEC2-Glucocorticoid Receptor transgene were used for LEC2 induction. Activity was induced in 8 day old seedlings for 1 or 4 hours with Dexamethasone application. Controls include uninduced transgenic plants and non-transgenic plants treated with Dex. Experiment Overall Design: Two biological replicates per treatment.
Project description:Arabidopsis seedlings were treated with 1 uM RALF peptide for 5 min to examine rapid protein phosphorylation changes. Phosphopeptides from the plasma membrane were identified and quantified using Orbitrap mass spectrometer.
Project description:Post-transcriptional gene regulation plays a significant role in the response to oxygen deprivation. Here, we utilized advances in next-generation sequencing technology to examine changes in transcriptional control, mRNA loading on to polysome, and regulation of ribosome activity during mRNA translation in 7-day-old Arabidopsis seedlings subjected to 2 hour hypoxia treatment.
Project description:Some soil bacteria promote plant growth, including Pseudomonas species. With this approach we detected significant changes in Arabidopsis genes related to primary metabolism that were induced by the bacteria. Pseudomonas G62 was applied to roots of 18 day-old Arabidopsis seedlings and the transcriptional profile of whole seedlings after 6 hours of treatment was analyzed.
